研究不同光敏剂诱导的光动力疗法对人白血病细胞的杀伤作用

Investigating the killing effect of photodynamic therapy induced by different photosensitizers on humanleukemia cell in vitro

目的研究并比较3种卟啉类光敏剂——血卟啉衍生物（HpD）、癌光啉（PsD007）和血卟啉单甲醚（HMME）诱导的光动力疗法（PDT）对白血病细胞K562的杀伤效应。方法以人白血病细胞K562为研究对象，分为对照组和PDT组，以梯度浓度的光敏剂与K562细胞共同孵育，经不同能量光照后，用噻唑蓝（M1Tr）法测定PDT对K562细胞的杀伤作用。结果与对照组相比，PDT对K562细胞有明显杀伤作用，并随着光敏剂浓度的增加和光照能量的增大，效果增强。PsD007一PDT和HMME．PDT的效果都明显优于HpD—PDT（尸〈0．05）；而当光敏剂质量浓度较大（25¨g／m1）或能量密度较大（7．2J^m2）时，PsD007一PDT的作用效果优于HMME—PDT。结论PDT对人白血病细胞K562具有明显的杀伤作用，其对细胞的抑制率具有显著的剂量效应关系；PDT对K562的杀伤效应与光敏剂种类有关，HpD．PDT的杀伤效果不如PsD007和HMME；在较高能量密度和较大光敏剂浓度的条件下，PsD007一PDT的效果优于HMME—PDT。

Objective To investigate and compare the killing effect of photodynamic therapy （PDT） induced by hematoporphyrin derivative （HpD）, hematoporphyrin monomethyl ether （HMME） and photocarcinorin （PsD007） on human leukemia cells K562 in vitro. Methods Human leukemia cells were cultured with serial concentrations of photosensitizers followed by irradiation of different dosage of laser light, then MT＇I＂ colorimetric assay was applied to measure the relative survival rate of PDT for the cells. Results Significant difference in the inhibitory between the PDT group and control group was observed （P〈0.05）. The survival rate of PDT for the cells elevated along with the increase in the concentration of sensitizer and dose of laser light. When the photosensitizer concentration was bigger （25 ~g/ml） or the energy density was bigger （7.2 J/cm~）, the effect of PsD007 was better than HMME, and they were significantly better than HpD （P〈0.05）. Conclusion PDT has significant killing effect on human leukemia cells K562, and its relative inhibitory rate appears to be correlated with the dose of sensitizer and laser light irritation. The effect of PDT is related to the photosensitizers. The effect of HpD-PDT is not as effective as PsD007 and HMME. On the conditions of higher energy density and larger photosensitizer concentration, the effect of PsDOO7-PDT is better than HMME-PDT.