西洋参水溶性蛋白的提取工艺研究

Study on Extraction Process of Water-solubility Protein from American Ginseng

目的:优化西洋参水溶性蛋白的最佳提取工艺。方法:采用单因素考察和L9(34)正交试验,以西洋参水溶性蛋白的提取率为考察指标,优化最优的提取工艺。结果:采用pH值为7的缓冲溶液,料液比(w/v)1∶15,浸提两次,每次12h为西洋参水溶性蛋白的最佳提取工艺。结论:优化得到的提取工艺合理,重复性良好,可为西洋参水溶性蛋白提取工艺的确定提供实验依据。

Objective ： To study the extraction process of water-solubility protein from American ginseng. Methods ： The orthogonal design L9 （34） was used and the water-solubility protein extraction rate was as the index, optimizing the best extraction process. Results ：The best process condition was that pH was 7,15 times amount of samples ,the extraction time was 12 hours ,extracting twice. Conclusion ： This process is the best and it can be used for extraction of water-solubility protein from American ginseng.