更年汤对围绝经期模型大鼠E_2、LH、FSH、GnRH的干预作用研究

Research on Intervention Effect of Gengnian Decoction on E_2,LH,FSH and GnRH of the Perimenopause Rats

目的:通过构建围绝经期大鼠模型,观察更年汤对模型大鼠内分泌相关指标的影响,探讨更年汤延缓卵巢衰老的作用机制。方法:将SD雌性大鼠随机分为更年汤组、教材方组、模型组、青年组、老年组各10只。前三组采用自然老化的围绝经期大鼠模型,青年和老年组为正常青年、老年模型。分别灌胃更年汤、教材方和生理盐水15日,于末次给药24h后麻醉,颈动脉取血5mL,剖取子宫、卵巢,观察血清卵泡刺激素(FSH)、黄体生成激素(LH)、雌二醇(E2)、促性腺激素释放激素(GnRH)和子宫、卵巢指数。结果:与模型组比较,治疗组、对照组E2含量均升高,治疗组有非常显著性差异(P<0.01);治疗组、对照组均能显著降低血清LH含量(P<0.01),组间比较有非常显著性差异(P<0.01);治疗组、对照组FSH含量均显著降低(P<0.01)组间比较无显著性差异(P>0.05);治疗组能显著降低血清GnRH含量(P<0.01),组间比较有非常显著性差异(P<0.01);治疗组、对照组均使卵巢、子宫指数升高,有非常显著性差异(P<0.01),卵巢组间比较差异无显著性差异(P>0.05),子宫组间比较有显著性差异(P<0.05)。结论:更年汤可明显提高模型大鼠E2水平,升高大鼠卵巢、子宫指数,降低血清FSH、LH、GnRH水平,从而达到延缓卵巢功能衰退的作用。

Objective：This study is proposed by constructing the menopausal transition model and observing the impact of the Medieinalbroth on the model rats＇ndocrine related index, aiming to explore the operating principle of Medicinalbroth. Methods：Divide the SD female rats randomly into 5 groups ： Medicinalbroth, Textbook, Model, Youth and Aged groups,with 10 in each group. The first three groups were naturally aging menopausal transition while the Youth and Aged groups were normal. Pour Medicinalbroth, textbook prescription and normal saline were separately given by intragastrie administration to Mediciualbroth, Textbook and Model groups. 24 hours after the last adiminstration, rats were given anesthesia. 5mL of blood samples from carotid artery was taken. Taking the womb and ovary, the variation of model rats FSH, LH, E2 , GnRH and weight of womb and ovary were observed. Results：Compared with the model group,the E2 content of Medieinalbroth and Textbook rised. The Medieinalhroth group had a very significant difference（P 〈0.01 ） ;LH content in the serum of the Medieinalbroth and the Textbook groups had signally re- dueed（P 〈 0. 01 ） and contents of each group differred from each other observably（ P 〈 0. 01 ） ; FSH content of the Medicinalbroth and the Textbook groups had signally reduced（ P 〈 0. 01 ） and contents of each group differred from each other indistinguishably（ P 〉 〈0. 05） ;GnRH content in the serum of the Textbook groups had signally reduced（ P 〈 0. 01 ）and contents of each group dif- ferred from each other observably（ P 〈 0. 01 ） ;weights of womb and ovary of the Medicinalbroth and the Textbook groups had sig- nally rised（ P 〈 0.01 ） and womb weights of each group differred from each other indistinguishably （ P 〉 〈 0.05 ） whereas ovary weights of each group differred from each other observably（ P 〈 0. 05 ）. Conclusion：Medicinalbroth can observably improve the E2 content of rat models,increase the womb and ovary weights of the rat models, decrease FSH,LH and GnRH contents in the serum and finally reach the goal of slowing ovary aging.