下肢静脉曲张与基质金属蛋白酶-9／金属蛋白酶抑制剂-2启动子区域基因多态性的关系

Polymorphism of metalloproteinases and inhibitors of matalloproteinases in patients with varicose veins

目的分析60例静脉曲张患者和60例正常人（无静脉曲张）基质金属蛋白酶-9（MMP-9）、金属赁门嗨抑制剂-2（TIMP-2）启动子区单核苷酸多态性（SNP），探讨其与静脉曲张血管重塑之间的关系．方法实验分静脉曲张患者组和对照组，静脉血5ml经枸橼酸钠抗凝，蛋白酶K消化，应用QIAGEN血液DNA抽提试剂盒提取基因组DNA，应用聚合酶链反应-限制性内切酶片段K腰多态性（PCR—RFLP）方法对MMP-9、TIMP-2启动予区SNPs值点进行基因分型。

Objective To study the relationship between human metalloproteinases （MMP-9）, endogenous tissue inhibitors of matalloproteinases （TIMP-2） promoters single nueleotide polymorphisms （SNP） anti varicose vascular remodeling in 60 cases of varicose vein and 60 normal controls. Methods Varicose vein group and control group were set up. We took 5 ml venous blood from every person of two groups, then extracted genomic DNA with QIAGEN blood extraction kit, and analyzed MMP-9/TIMP-2 promoter regiorl SNPs genotyping by polymerase chain reaction-restriction fi＇agment length polymorphism （ PC R-RFLP）. Results In varicose vein group, there were 54 cases of homozygosity and 6 cases of hetero- zygote fi^r I）NA/MMP-9, and there were 32 cases of homozygosity and 28 eases of heterozygote for DNA/ TIMP-2. In control group, there were 44 cases of homozygosity and 16 cases of heterozygote for DNA/ MMP-9, and there were 43 cases of homozygosity and 17 cases of heterozygote for DNA/TIMP-2. Conclusion The results suggest that gene polymorphisms in the MMP-9 and TIMP-2 promoter region are associated with varicose veins.