摘要
目的观察富含脯氨酸的蛋白酪氨酸激酶2(Pyk2)对人大肠癌细胞生长和肺转移能力的影响。方法利崩人大肠癌细胞株SW480,通过止义及反义eDNA稳定转染技术,分别构建Pyk2高表达和低表达的细胞SW480-Pyk2-和SW480-Pyk2-,并以未转染质粒和转染空质粒的SW480细胞作为对照。分别建辽裸鼠皮下种植瘤和肺转移模型。6周后,切取皮下种植瘤体并称重;切取肺转移标本,在显微镜下计数转移灶数目。结果Westernblot法证实Pyk2高表达、低表达及宅质粒细胞构建成功;SW480-Pyk2’组(MeanRank=6.19)皮下种植瘤质量明显低于SW480组(MeanRank=18.69)和SW480-Vector组(MeanRank=14.12),而SW480-PI,k2-组(MeanRank=27.00)日月显高于SW480组和SW480-vector组,差异有统计学意义(x2=20.646,P〈0.05);SW480-Pyk2’组(MeanRank=6.62)肺转移灶数目明显少于SW480组(MeanRank=16.06)和SW480-veetor组(MeanRank=14.94),SW480-Pyk2组(MeanRank=28.38)肺转移灶数目明显多于SW480组和SW480-vector组,差异有统计学意义(x2=21.948,P〈0.05);并且种植瘤质量和肺转移灶数与Pyk2的表达量均旱明显负相关(r=-0.776,-0.777,P〈0.05)。结论Pyk2在裸鼠体内能够抑制大肠痈细胞的生长和肺转移能力。
Objective To investigate the effects of the proline-rich tyrosine kinase 2 ( Pyk2 ) on the growth and the hematogenous metastasis of the human colorectal cancer SW480 cells. Methods SW480 cells were stably transfected with empty vector (pcDNA4-A) , vector containing Pyk2 + or Pyk2 , respeclively. Cells from each group (SW480-Pyk2+, SW480, SW480-vector and SW480-Pyk2 cells) were inoculated subcutaneously into g nude mice ( BALB/C nu/nu) respectively. Six weeks later, the sub- cutaneous tumors were harvested and weighed. Lungs were harvested 6 weeks after nude mice lung metasta- sis model was constructed. Then the lung metastatic amounts were counted by microscopy. Results The mass of the subcutaneous tumors in SW480-Pyk2 + group (Mean Rank = 6. 19) was less than other groups ( SW480 18.69, SW480-vector 14. 12), and that in SW480-Pyk2 group ( Mean Rank = 27.00) was more than other groups (X~ = 20. 646, P 〈 0. 05 ). The lung metastatic amounts in SW480-Pyk2 + group ( Mean Rank = 6.62) were the least, those in SW480-Pyk2- group ( Mean Rank = 28.38) were the most, and those in the remaining two groups ( SW480 16. 06, SW480-vector 14. 94) were moderate (X2 = 21. 948, P 〈 0. 05 ). The expression of Pyk2 was highly negatively ton'elated with the mass of the tumors and lung metastalie amounts ( r = - 0. 776, - 0. 777, P 〈 0.05 ). Conclusion Pyk2 could inhibit the growth and the hmg metastasis of SW480 cells in vivoin nude mice.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第9期1750-1752,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目(30572105)
关键词
结直肠肿瘤
蛋白酪氨酸激酶2
血行转移
Colorectal neoplasms
Proline-rich tyrosine kinase 2
Hematogenous metastasis