微囊化罗非鱼胰岛移植治疗小鼠糖尿病

Grafting of microencapsulated tilapia islets to treat diabetic mice

目的观察微囊化罗非鱼胰岛移植对糖尿病小鼠的治疗作用。方法胶原酶消化分离罗非鱼胰岛。链脲霉素小鼠腹腔内注射，建立药物性糖尿病小鼠模型。微囊包裹罗非鱼胰岛后，移植入糖尿病小鼠腹腔内，即微囊化组（12只）；设立生理盐水（10只）为对照组。比较移植后两组糖尿病小鼠的存活率；动态观察小鼠血糖的变化。并对移植物进行动态病理检查。结果移植后4周微囊化组受体存活率为40％，明显高于生理盐水组（P〈0．05）。微囊化组糖尿病小鼠血糖降低，持续（15．70±3．09）d，与生理盐水组比较差异有统计学意义（P〈0．05）。微曩化组在移植后7d，可从受体腹腔中收集到形态完整的微囊，其内罗非鱼胰岛活力良好。移植后14d可见微囊形态依然完整，其中罗非鱼胰岛大多已死亡。结论微囊化包裹的罗非鱼胰岛腹腔内移植能降低糖尿病小鼠血糖，提高糖尿病小鼠的存活率。

Objective To observe the effect of microencapsulated tilapia islets transplantation to diabetic mice. Methods Tilapia islets were harvested, isolated and encapsulated with the sodium alginate. The diabetic model in balb/c mice were made by intraperitoneal injection of STZ. Diabetic mice were divid- ed randomly into 2 groups： microencapsulated group （n = 12）, and normal saline （NS） group （n = 10）. Four-week survival rate was observed. The blood glucose levels were tested and compared after transplanta- tion. The samples were examined histologically after hematoxylin and eosin （HE） staining. Results Four- week survival rate in microencapsulated group was higher than NS group （ P 〈 O. 05 ）. The microencapsuia- ted tilapia islets could maintain normoglycemia for （ 15.70 -＋ 3.09） days in diabetic mice. At the 7 day af- ter transplantation, no infiltrating inflammatory cells were found in the microcapsules, and islets still re- mained intact. At the 14 day after transplantation, some islets formed the cluster. The microcapsules still remained round and intact, but islets in the mierocapsules presented necrosis, and microcapsules were in- filtrated and surrounded by massive inflammatory cells and fibrin. Conclusion The intraperitoneal trans- plantation of alginate-encapsulated tilapia islets could maintain blood glucose levels in diabetic mice and improve the survival rate of diabetic mice.