血管活性肠肽在前列腺腺癌组织及细胞中的表达及临床意义

Expression and significance of vasoactive intestinal peptide in prostatic carcinoma tissue and cells

目的观察血管活性肠肽（VIP）在前列腺癌组织及细胞中的表达，探讨VIP在前列腺腺癌发生及发展中所起的作用。方法通过免疫组织化学法检测20例前列腺增生组织、39例前列腺癌组织中VIP蛋白的表达，通过逆转录-聚合酶链反应（RT-PCR）方法检测15例前列腺增生组织、18例前列腺癌组织和培养的前列腺癌细胞中的mRNA的表达。结果前列腺癌组织和培养的前列腺癌细胞中均有VIPmRNA表达，且前列腺癌组织中VIPmRNA要高于前列腺增生组织，差异有统计学意义（P〈0．05）。前列腺癌组织出现VIP免疫反应阳性细胞表达率（69．23％）高于前列腺增生组织（30．00％），差异有统计学意义（x2=8．255，P〈0．01）。前列腺癌组织中VIP免疫反应阳性细胞表达率病理分化程度的降低、临床分期的增高，呈逐渐上升趋势，差异有统计学意义（X2=6．957、P〈0．05；x2=6．184、P〈0．05），且同病理分化程度、临床分期明显相关（r=0．390、P〈0．01；r=0．368、P〈0．01）。结论前列腺癌组织中VIP表达明显高于前列腺增生组织，VIP可能在前列腺癌的发生和发展中起重要作用。

Objective To study the expression of vasoactive intestinal peptide （VIP） in prostatic adenocareinoma and evaluate the role of TP in tumor genesis and progress of prostatic adenoearcinoma. Methods The expression of VIP protein in 20 cases of benign prostatic hyperplasia and 39 cases of prostatic adenoearcinoma was detected by using immunohistochemieal staining. The expression of VIP mRNA in 15 cases of benign prostatic hyperplasia, 18 cases of prostatic adenocarcinoma and LNCaP was detected by using reverse transcfiption-polymerase chain reaction （RT-PCR）. Results The expression of VIP mRNA in prostatic adenocareinoma and LNCaP was detected, which was higher than in benign prostatic hyperpla- sia（ P 〈 0. 05 ）. The positive expression rate of VIP protein was higher in prostatic adenocarcinoma （69.23%） than that in benign prostatic hyperplasia （30. 00% ） （X2 = 8, 255 ,P 〈0. 01 ）. There was sig- nificant difference in the VIP expression among the differentiated pathological grades and clinical stages （X2 = 6. 957, P 〈 0. 05 ;X2 = 6. 184, P 〈 0. 05 ）, and there was a correlation between VIP expression and the the differentiated pathological grade and clinical stage （ r = 0. 390, P 〈 0. 01 ; r = 0. 368, P 〈 0. 01 ）. Conclusion The prostatic adenocarcinoma cells can secret VIP, indicating that VIP may play an important role in tumor genesis and progress of prostatic adenoeareinoma.