摘要
目的:寻找一种新型的转染效率高,毒性低的非病毒基因载体。方法:通过化学方法合成Polyimine-MPEI,然后以不同质量比包裹绿色荧光蛋白质粒,检测在COS-7细胞中的转染效率和毒性。结果:在比例从5到100之间,转染效率均比较理想,能达到1.00E+07以上,Polyimine-MPEI的毒性也很小,细胞的生长率均在80%以上,明显高于PEI25KDa对照组。结论:Polyimine-MPEI是一个很有研究前景的聚合物载体,具有高转染效率低毒性的特点,可以通过延长反应时间,增加分子量,增大转染能力。
Objective: To find a novel nonviral gene vector with a high transfection efficiency and low cytotoxicity. Methods: Polyimine-MPEI is synthesized, then packed with luciferase gene at different mass ratio. The transfection efficiency and cytotoxitity of this polyplex was detected. Results: From mass ratio 5 to 100, the transfection efficiency of the polyplex was above 1.00E+07 and it was relatively ideal. Its cytotoxicity was lower comparing with the PEI25KDa control group and the cell viability was all above the 80%. Conclusions: Polyimine-MPE1 is a polycation vector with a bright research prospect, which has high transfection efficiency and low cytotoxicity. Pro- longing the synthesis time and making its molecular weight higher can increase the transfection efficiency.
出处
《现代生物医学进展》
CAS
2012年第24期4611-4613,共3页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(200801198)