慢性阻塞性肺疾病大鼠模型中痩素及白介素-8的表达分析

The Analysis of Leptin and Interleukin-8 Expression in Rat Models of Chronic Obstructive Pulmonary Disease

目的:观察COPD大鼠模型中瘦素(leptin)、白细胞介素8(IL-8)的表达情况,分析其相关性,探讨leptin在COPD发生发展中的作用及意义。方法:36只雄性SD大鼠随机分为①健康对照组;②COPD模型1组:分别于第(1、14)d经气管内注入内毒素200ug,熏5%香烟(第1、14d除外),2h/d,共4周;③COPD模型2组:单纯熏5%香烟2h/d,共12周。观察肺组织病理变化,免疫组化法测定leptin、IL-8在支气管肺组织的表达情况,放免法测定血清leptin及IL-8浓度。结果:细胞因子leptin及炎性因子IL-8在支气管肺组织阳性表达。LPS联合熏烟诱导COPD1组支气管肺组织中leptin(52.67±04.72)和IL-8(59.56±3.94)表达较单纯熏烟诱导COPD2组leptin(38.89±2.57)和IL-8(55.22±3.42)表达明显升高,P<0.05,两组COPD大鼠模型支气管肺组织中leptin及IL-8表达较正常对照组leptin(16.90±1.52)和IL-8(28.00±4.24)表达均明显增高,P<0.05,COPD1组血清中leptin(3.26±0.95)ng/mL和IL-8(107.51±13.38)pg/mL较COPD2组中leptin(2.42±0.69)ng/mL和IL-8((94.07±11.20)pg/mL明显增高,P<0.05,两组COPD大鼠模型血清中leptin及IL-8浓度较正常对照组leptin(0.95±0.56)ng/mL和IL-8(39.48±6.35)pg/mL浓度显著升高,P<0.05。血清中Leptin与IL-8表达水平呈显著正相关(r值分别为0.72 0.67 0.84均P<0.05)。经过q检验,两两之间比较均有统计学意义。结论:leptin和IL-8均参与COPD炎症反应过程,并且具有相关性,LPS促进二者的表达。

Objective： To investigate the expression matter of leptin and IL-8 in rat models of COPD, and analyse their relevance, investigate the value of leptin in the development of COPD. Methods： 36 Male SD rats were randomly divided into 3 groups： （1） Control group： untreated rats; （2）COPD 1： rats were intratracheally administered LPS （200 μg） on d 1 and 14 and exposed to 5% smoke for 2 h daily for 4 consecutive weeks; （3） COPD2： rats were exposed to 5% smoke for 2 h daily for 12 consecutive weeks. To observe the pathological changes of the lung tissues, and take immunohistochemistry to determine leptin and IL-8 levels expressed in bronchial lung tissue. Serum leptin and IL-8 concentration was examined by radio-immunity method. Results： The cell factor leptin and inflammatory factor IL-8 in bronchial lung tissue were positive expressed. The expression ofleptin （ 52.67± 04.72）and IL-8 （59.56± 3.94） in COPD 1 group was higher than that in COPD2 group leptin（38.89± 2.57） and IL-8（55.22± 3.42）, P〈0.05; Both leptin and IL-8 expression in two COPD model groups were higher than that in the normal group （16.90± 1.52） and （28.00± 4.24）, P〈0.05; In the COPD1 group, serum leptin level （3.26± 0.95） ng/mL and IL-8（107.51± 13.38） levels were markedly higher than that in the COPD2 group（2.42± 0.69）ng/ml and （94.07 ± 11.20） pg/mL, P 〈 0.05, and these levels in both COPD groups increased dramatically compared with that in controls （0.95± 0.56）ng/mL and IL-8 （39.48± 6.35） pg/mL, P〈0.05. There was negative correlation between leptin and IL-8 expression（r=0.72 0.67 0.84 all P〈0.05）in blood. The comparison between the two groups had statistical significance after inspection. Conclusion： Leptin and IL-8 play important roles in the occurrence and development of COPD, which is correlated with each other. In addition, LPS facilitates the expression of leptin and IL-8.