儿童血清脂肪细胞因子与肥胖和非酒精性脂肪肝的关联

Association between serum IL-6,PAI-1,RBP-4 levels and obesity,nonalcoholic fatty liver disease in children

目的研究白细胞介素-6(IL-6)、血浆纤溶酶原激活物抑制剂1(PAI-1)、视黄醇结合蛋白4(RBP-4)水平在非酒精性脂肪肝(NAFLD)、肥胖儿童血清中的变化,为儿童NAFLD血清学标志物的发现提供依据。方法采用双抗体夹心酶联免疫吸附法,测定儿童血清IL-6、PAI-1、RBP-4水平。结果体重正常组与肥胖组IL-6、PAI-1水平差异均无统计学意义(P值均>0.05),体重正常组RBP-4水平显著高于肥胖组(P<0.01);NAFLD组的IL-6水平明显高于非NALFD组(P<0.01),NAFLD组的RBP-4水平显著低于非NAFLD(P<0.01);未见NAFLD组与非NAFLD组之间PAI-1水平存在差异(P>0.05)。NAFLD分度与IL-6水平呈正相关(β=0.168,P=0.013),与RBP-4呈负相关(β=-0.262,P=0.001)。结论肥胖儿童RBP-4水平低于正常体重儿童;IL-6及RBP-4与儿童NAFLD有关,可能在儿童脂肪肝的发病过程中起一定作用。

Objective To study the relationship between serum IL -6, PAI- 1 and RBP -4 levels and obesity, nonalcoholic fatty liver disease in children. Methods The quantitative enzyme linked sandwich immunoassay （ ELISA ） was used to detect the serum concentrations ofIL-6（n =217} , PAI- 1 （n =213} and RBP-4（n = 157）. Results No significant differences of IL - 6 or PAl - I levels were found between obesity and normal weight children （ P = 0.44 and P = 0.40, respectively } , while normal weight children had higher RBP- 4 level than obese children（P 〈 0.01 }. Children with NAFLD had higher IL- 6 but lower RBP -4 level compared to non-NAFLD children（ P 〈0.01 }. No significant difference of PAI- 1 level was found between NAFLD and non-NAFLD children { P 〉 0.05）. NAFLD degrees were positively related with IL- 6 level and negatively related with RBP -4 （13 =0. 168, P=0.013; fl= -0.262, P =0.001, respectively）. Conclusion RBP-4 may play a role in childhood obesity; IL- 6 and RBP -4 are associated with NAFLD in children and may involve in pathological process in ehildhood NAFLD.