静电纺丝天然-人工合成聚合物复合纳米纤维及其对细胞黏附、增殖和迁移的影响

Electrospun natural-artificial synthetic polymer blended nanofibers and their influence on cellular adhesion,proliferation and migration

生物材料组成成分对细胞生物功能有不同的影响。利用静电纺丝技术制备了基于聚己内酯(PCL,polycaprolactone)的不同天然蛋白、多糖(丝素蛋白(SF,silk fibroin)、透明质酸(HA,hyaluronicacid))的混合组分纳米纤维,采用了扫描电镜和接触角对纳米纤维进行基础表征。同时,进一步考察了纳米纤维作为组织工程支架的可行性。研究结果表明SF组分能增加材料的可纺性,有利于细胞的前期黏附,并能够促进细胞增殖。HA组分可以改善材料的亲水性,增加细胞伪足并促进细胞迁移。重要的是,PCL/SF/HA纳米纤维能同时结合SF和HA的优点,有望在组织工程领域得到应用。

The scaffold compositions played different roles on cellular biological functions.In this study,we developed polycaprolactone（PCL） based nanofibers composed of natural protein and polysaccharides（hyaluronan（HA）,silk fibroin（SF）） via electrospinning technology.These nanofibers were characterized by scanning electron microscopy（SEM） and contact angle.Furthermore,we evaluated the potential of different composite nanofibers as tissue engineering scaffolds.In vitro cultivation of human primary skin fibroblasts on the SF-based scaffolds showed a significant increase in cell proliferation.A significant number of cells were found to have well-developed cytoskeleton on PCL/SF nanofibers.Addition of HA component transformed current PCL and PCL/SF components into hydrophilic fibers.Importantly,HA-based scaffolds significantly enhanced cell filopodia protrusions and migration in vitro.PCL/SF/HA nanofibers could combine the advantages of SF and HA.These findings suggest that such multiple blended nanofibers may offer possibilities to tissue engineering application.