红花注射液致Ⅰ型过敏反应试验

Study on the type I allergic reaction of safflower injection

目的:研究红花注射液在全身主动过敏试验和被动皮肤过敏试验中是否出现过敏反应。方法:豚鼠分别腹腔注射红花注射液5 mL.kg-1,0.9%氯化钠注射液5 mL.kg-1及牛血清白蛋白生理盐水注射液5 mL(40 mg).kg-1,qod,连续3次进行致敏。末次致敏后14 d各组静脉单次注射2倍剂量的以上相应受试物进行激发,观察30 min内是否有过敏反应;SD大鼠皮内注射相应抗血清0.1 mL被动致敏48 h后,分别静脉注射红花注射液5 mL.kg-1,0.9%氯化钠注射液5 mL.kg-1和牛血清白蛋白生理盐水溶液5 mL(40 mg).kg-1及1%伊文思蓝溶液共1 mL进行激发,30 min后处死动物,观察注射点皮肤蓝斑直径。结果:红花注射液组致敏期间豚鼠未出现任何异常反应,给予激发剂量后红花注射液组其中一个批次出现异常反应,但未出现豚鼠死亡;红花注射液组和牛血清白蛋白溶液组大鼠背部皮肤内层均出现蓝斑。结论:红花注射液组全身主动过敏反应和被动皮肤过敏均为阳性,红花注射液具有一定的致敏性。

OBJECTIVE To study the allergic reaction of safflower injection with active systemic anaphylaxis test and passive cutaneous anaphylaxis test.METHODS Guinea pigs were injected respectively with safflower injection（5 mL·kg^-1）,sodium chloride injection（5 mL·kg^-1） or bovine serum albumin saline injection [5 mL（40 mg）·kg^-1],once every other day,continuously for three times.Each group was performed by injecting a single intravenous injection of double dosages of test substance,after 14 d from the end time of sensitization,to observe whether allergic reactions occurred within 30 min;SD rats were received 0.1 mL of antiserum injection for inducing passive sensitization,after 48 hours,respectively injected with safflower injection（5 mL·kg^-1）,sodium chloride injection（5 mL·kg^-1） or bovine serum albumin saline injection [5 mL（40 mg）·kg^-1] and 1 mL of 1% Evans blue for performing.After 30 min the rats were sacrificed,and the diameter of blue spots on the dorsal skin were observed.RESULTS There was no any abnormal reaction during sensitization in the safflower injection group.Adverse reactions had been found in one batch of safflower injection group,but death was not observed.Blue spots were found on the SD rats＇ dorsal skin of the groups of safflower injection and bovine scrum albumin.CONCLUSION The test of active systemic anaphylaxis and passive cutaneous anaphylaxis of safflower injection were proved positive reactions.Safflower injection could increase the sensitization to allergic reaction.