星状神经节阻滞对内毒素血症小鼠炎性细胞因子水平及死亡率的影响

Effects of SGB on serum cytokines and mortality of mice with endotoxemia

目的:探讨星状神经节阻滞(SGB)对内毒素血症小鼠血清炎性细胞因子及死亡率的影响,明确SGB可否成为系统性炎性反应综合征(SIRS)简单有效的辅助治疗手段。方法:内毒素血症小鼠分为SGB治疗组(颈部注射利多卡因)及对照组(颈部注射生理盐水),观察两组动物腹腔注射脂多糖(LPS)后2、7、14d血清TNF-α、IL-1β、IL-6的变化及2、4、7、10、15、20d累积死亡率的变化。结果:SGB治疗使内毒素血症小鼠2、7、14d的血清炎性细胞因子TNF-α、IL-1β、IL-6水平显著下降;注射LPS后2、4、7、10、15、20d的死亡率显著降低。结论:SGB可抑制内毒素血症小鼠过度的炎性反应,降低内毒素血症小鼠的死亡率,从而成为有益于SIRS治疗的简单有效的新手段。

Objective To determine the feasibility of stellate ganglian block （SGB） therapy for the treatment of mice with endotoxemia. Methods Kun ming mice injected with 2 mg/kg Lipopolysaccharide （LPS） intraperitonealy were divided into control group （untreated by SGB） and SGB group （treated daily by SGB）. The roles of SGB were evaluated by comparing mortality and serum TNF-α,IL-1β,IL-6 levels in each group. Results Compared with control group, Mortality in SGB group decreased significantly at 2, 4, 7, 10, 15, 20 d after injecting LPS intraperitonealy. SerumTNF-α,IL-1β,IL-6levels decresed obviously in SGB group than that in control group. Conclusions These results indicate that SGB is a effective central therapeutic approach for treatment of endotoxemia. SGB can regulate systemic infammatory responses, which maybe mechanisms for effectiveness of SGB on mortality in mice with endotoxemia.