8-羟基脱氧鸟苷酸和血管内皮生长因子与糖尿病肾病的关系

Relationship of serum 8-OHdG and VEGF with diabetic nephropathy in diabetics

目的探讨DNA氧化损伤标志物血清8-羟基脱氧鸟苷酸(8-OHdG)和血管内皮生长因子(VEGF)与糖尿病肾病(DN)的关系。方法随机检测71例DN患者,73例糖尿病患者及健康对照者47名,采用酶联免疫吸附实验(ELISA)法测定其血清8-OHdG、VEGF水平24h微量白蛋白,并与47名健康对照者进行比较。结果 8-OHdG和VEGF水平DN组显著高于糖尿病组(P<0.01),糖尿病组高于正常对照组(P<0.05)。大量蛋白尿组明显高于微量蛋白尿组(P<0.05)。HbA1c>10%组明显高于糖化血红蛋白<9%组(P<0.05)。结论 DN与血清8-OHdG、VEGF有关,氧化应激和VEGF参与DN发生发展过程。

Objective To investigate the relationship of serum 8-hydroxy-guanine （8-OHdG） and vascular endothelial growth factor （VEGF） with diabetic nephropathy （DN）. Methods The levels of serum 8-OHdG, VEGF, and 24 h albuminuria were measured by ELISA in 71 DN patients, 73 T2DM patients, and 47 healthy people. The results were compared to observe the difference among them. Results The levels of serum 8-OhdG and VEGF in the diabetic nephropathy patients were significantly higher than in the T2DM patients （P〈0.01）. The levels of serum 8-OhdG and VEGF in the T2DM patients were higher than in the normal control group （P〈0. 05）. The levels of serum 8-OhdG and VEGF in the macroalbuminuria group were significantly higher than in the microalbuminuria group （P〈0. 05）. The levels of serum 8-OhdG and VEGF in the HbA1 c〉10% group were significantly higher than in the HbA1 c〈9% group （P〈0.05）. Conclusion The diabetic nephropathy is related with serum 8-OHdG and VEGF. Oxidative stress and VEGF play important roles in the pathogenesis of diabetic nephropathy.