摘要
为获得γ-氨基丁酸(γ-aminobutyric acid,GABA)的高产菌株,对乳酸片球菌进行紫外线和亚硝基胍诱变及二者的复合诱变处理。以正突变为标准确定的最佳诱变条件为:紫外照射距离25cm,照射时间20s;NTG终浓度为0.3mg/mL,处理时间60min。诱变后获得了1株突变株UN-27,经连续传代10次,遗传性状稳定,平均GABA产量为5.017g/L,较初始菌株的1.062g/L提高了4.72倍。
In order to achieve the higher yield of GABA, Pediococcus acidilactici was mutagenized with ultraviolet （UV）, nitrosoguanidine （NTG） and UV-NTG. According to the forward mutation, the optimum mutagenesis conditions are： 25 cm range of UV irradiation, 20 s irradiation, 0.3 mg/mL final concentration, and 60 min mutagenizing. The mutant strain named UN-27 was obtained through the mutagenesis, which remained the GABA-producing capacity stability after 10 times breeding. The average output of GABA is 5.017 g/L, which has been improved 4.72 times than the initial strain＇s 1.062 g/L.
出处
《食品科技》
CAS
北大核心
2012年第9期16-19,共4页
Food Science and Technology
基金
黑龙江省教育厅2010年度科学技术研究(面上)项目(11551361)
