姬菇多糖对酒精所致急性肝损伤小鼠肾脏保护的研究

Protective effect of polysaccharides from Pleurotus cornucopiae on acute alcoholic hepatic injury of kidney in mice

目的:研究热水浸提法从姬菇中提取姬菇多糖(PCNP)对酒精所致急性肝损伤小鼠肾脏的影响。方法:小鼠被随机分为空白对照组、模型组、多糖各剂量组(100、200、400mg/kg bw),连续灌胃30d后,除空白组外,给予50%浓度的乙醇溶液建立动物急性肝脏损伤模型。12h后,动物脱臼处死后取血液和肾脏测定各项抗氧化指标。结果:与模型组相比,多糖各剂量组能降低血清TG和肾脏MDA含量,提高肾脏SOD、CAT活性、GSH-Px活性及GSH含量。结论:PCNP对酒精所致小鼠肾损伤具有明显保护作用。

Objective： The present study investigated the protective effect of polysaccharide from Pleurotus cornucopiae （PCNP） on acute alcoholic hepatic injury of kidney in mice. Methods： The mice were randomly divided into blank control group, alcoholic model groups. And also divided into alcoholic model groups were administered by gavage with BIFENDATE（150 mg/kg bw） and PCNP （100, 200, 400 mg/kg bw） for 30 days prior to the administration with dose of 50% alcohol （12 mL/kg bw） except blank control group. 12 hours after the last alcohol treament, all mice were dislocation death to assay activities of antioxidant capabilities in kidney and TG in blood. Results： Compared with the model group, the contents of MDA in kidney and TG in serum were obeviously decreased also the contents of GSH and activities of SOD, CAT, GSH-Px, obeviously increased in the PCNP treatment groups. Conclusions： PCNP protect the acute alcoholic hepatic injury of kidney induced by alcohol in mice.