利用薯蓣皂素生产废水发酵生产s-腺苷蛋氨酸的初步研究

Using wastewater of Dioscorea Zingiberensis C.H.Wright to produce S-adenosyl-L-methionine by fermentation

通过采用传统的硫酸水解工艺制得皂素废水,然后采用3种不同方式对废水进行预处理,实现了利用废水发酵生产s-腺苷蛋氨酸,同时降低废水COD值的目的。实验表明:3种不同的预处理方式对s-腺苷蛋氨酸的产量影响不同,将纯糖液的s-腺苷蛋氨酸产率记作100%,处理1,只调节废水pH发酵得到的s-腺苷蛋氨酸产量为7.75171g/L,达到纯糖液发酵的12.37%,同时废水COD去除率达35.11%;处理2,先调节废水pH然后进行活性炭吸附之后发酵得到s-腺苷蛋氨酸产量为4.29515g/L,达到纯糖液发酵的6.86%,同时废水COD去除率达71.68%;处理3,先进行活性炭吸附然后调节其pH之后发酵得到的s-腺苷蛋氨酸的产量为10.21076g/L,达到纯糖液发酵的16.30%,同时废水COD的去除率达到65.74%。从工业生产成本的角度出发,处理3的方式为最佳方式。研究实现了废水中有用资源的再利用,产生了明显的经济效益和环境效益,为工业化生产提供了理论支撑。目前该项研究在国内外未见报道。

Got the wastewater of Dioscorea Zingiberensis C.H.Wright using the traditional process technology to hydrolyze, and pretreated with the wastewater in three kinds of ways, achieving the purpose of producing S-adenosyI-L-menthionine by fermentation meanwhile reducing the COD value of wastewater. The result indicated that the three different pretreatment ways had influenced the yield of S-adenosyI-L-menthionine. Defining the yield ratio of S-adenosyI-L-menthionine fermenting in pure sugar liquid was 100%. Process one： only adjusted pH, the fermentation yield of S-adenosyI-L-menthionine reached 7.75171 g/L, accounting 12.37% of pure sugar fermentation medium and removal rate of COD was 35.11%; Process two, adjusted pH first and then absorbed with activated carbon, the fermentationyield of S-adenosyI-L-menthionine reached 4.29515 g/L, accounting 6.86% of pure sugar fermentation medium and removal rate of COD was 71.68%; Process three, absorbed with activated carbon first and then adjusted pH, the fermentation yield of S-adenosyI-L-menthionine reached 10.21076 g/L, accounting 16.30% of pure sugar fermentation medium and removal rate of COD was 65.74%. Considering the cost in the production, process three is the best. Recycling the valuable resurces of wastewater and having achieved good environmental and economical benefits and providing theoretical support for industrial production. At present the study had not seen the report at home and abroad.