摘要
为选择适合奶粉材料进行分子标记检测的DNA提取方法,以市售奶粉为材料,采用6种不同的方法提取基因组DNA,并比较这6种方法的提取效果.结果表明,除异丙醇沉淀法和十二烷基硫酸钠法(SDS法)外,其他4种方法提取的基因组DNA均适用于聚合酶链式反应(PCR)检测.同时,结合基因组DNA的纯度和质量浓度,明确奶粉基因组DNA提取方法的优劣依次为:热解法、异硫氰酸胍法、高盐低pH法和十六烷基三甲基溴化铵法(CTAB法).
There are 6 methods,pyrolysis,high-salt low-pH,isopropyl alcohol,CTAB,SDS or GuSCN,used in the extraction of milk powder genomic DNA for the molecular marker analysis of high quality and sufficient samples.To find out the better method of them,the effects of different methods were compared by detecting the DNA by optical density,agarosegel electrophoresis and polymerase chain reaction（PCR）.The results showed that the genomic DNA extracted by all methods except for isopropyl alcohol and SDS method could be used in PCR reaction.Meanwhile,the better DNA concentration and purity could be gained by different methods in the order of pyrolysis,GuSCN,High-salt low-pH,CTAB.These methods are simply to operate,fast to gain results,and suitable for the extraction of total DNA from milk powder.
出处
《哈尔滨工业大学学报》
EI
CAS
CSCD
北大核心
2012年第8期73-75,129,共4页
Journal of Harbin Institute of Technology
基金
哈尔滨工业大学科研创新基金资助项目(HIT.NSRIF.2012081)
四川省应用基础研究(2009JY0101)
关键词
奶粉
基因组DNA
提取方法
聚合酶链式反应
milk powder
genomic DNA
extraction method
polymerase chain reaction（PCR）
