新疆褐牛核心群母牛蜘蛛腿综合征的遗传检测

The Genetic detection of arachnomelia syndrome in the breeding group of Xinjiang brown cattle

以173头新疆褐牛核心群母牛血样为基础,采用荧光引物PCR产物毛细管电泳法结合PCR产物直接测序法检测蜘蛛腿综合征隐性基因。结果显示,荧光引物PCR产物毛细管电泳法检测的173份血样DNA片段均在246～250bp出现单一信号峰。其中,21份PCR产物直接测序未出现牛蜘蛛腿综合征SUOX基因致病突变G碱基插入导致的叠峰现象,序列比对显示测序检测未发现突变的杂合子个体,22种方法检测结果一致。结果表明,2种检测方法所检测的新疆褐牛核心群母牛个体均为纯合基因型,没有发现牛蜘蛛腿综合征SUOX致病等位基因的存在或携带该致病基因的个体,说明该研究群体不存在传播牛蜘蛛腿综合征的风险。

173 blood samples from core group of Xinjiang brown cows were used in this study. Using capillary elec- trophoresis combined with direct sequencing of PCR products tests arachnomelia syndrome mutation. All of the 173 samples tested by capillary electrophoresis showed a single signal peak among the 246-250 bp. 21 samples were fur- ther sequenced and no overlapping peaks caused by the G base insertion in SUOX was found. No heterozygous indi- viduals were detected by sequence alignment. The results of the two methods were consistent. The result showed the core group of Xinjiang brown cows is homozygous genotype and no SUOX allele exists,indicating that there is no risk of transmission of AS in this bovine group.