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草原红牛CAPN1基因mRNA表达特性分析 被引量:3

Expression characteristic of CAPN1 gene at mRNA level in Chinese red steppe
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摘要 为了进一步揭示CAPN1的功能,本研究采用实时荧光定量PCR技术对草原红牛初生牛与成年牛心脏、肝脏、脾脏、肺脏、肾脏、瘤胃、十二指肠、背最长肌8种组织中的CAPN1mRNA表达水平进行了分析。结果表明:CAPN1基因在组织中普遍表达,但表达量存在差异。且成年牛的CAPN1基因在肺脏和瘤胃组织中的表达显著高于初生牛(P<0.05),成年牛在肝脏、脾脏、肾脏和背最长肌组织中CAPN1基因的表达水平极显著高于初生牛(P<0.01)。 The present study was designed to detect the expression of CAPN1 mRNA in the heart,liver, spleen,lung, kidney,rumen,duodenum,longissimus dorsi of newborn and adult Chinese Red Steppe by Real time RT-PCR. The results showed that CAPNlmRNA was expressed in all of eight tissues detected. We also found that CAPNlmRNA expression level was higher in lung and rumen of adult than newborn(P〈0.05) ;but the expression level of CAPN1 mRNA expression level was significant higher in liver, spleen, kidney and longissimus dorsi of adult than newborn(P〈0.01).
出处 《中国兽医学报》 CAS CSCD 北大核心 2012年第9期1302-1304,共3页 Chinese Journal of Veterinary Science
基金 国家肉牛牦牛产业体系(nycytx-38) 肉用草原红牛培育(吉财农指[2008]883号)
关键词 中国草原红牛 实时荧光定量PCR CAPN1基因 Chinese Red Steppe Real-time quantitative PCR CAPN1
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