经营田鼠染色体核型与G带分析

Analysis of the karyotype and G banding of chromosomes in Microtus limnophilus

采用骨髓法制备经营田鼠染色体标本片,并对其染色体核型和G带进行分析。结果表明,经营田鼠体细胞染色体数为2n=38,雄性染色体核型由18对常染色体和1对异配型性染色体XY组成,雌性为18对常染色体和1对同配型性染色体XX组成。1～9号为端着丝粒(t)染色体(包括Y染色体),10号为近端着丝粒(st)染色体,11～18号为中央着丝粒(m)染色体(包括X色体)。19对染色体共分布有370条G带(雄性365条),其中深带190条,浅带180条。因此,经营田鼠的染色体数目、G带具有明显种的特征,与其他鼠类不同。

The research is to study the karyotype and G-banding pattern of the chromosomes in Microtus limnophi- lus. The Microtus limnophilus chromosome samples were prepared by the use of bone marrow method test,and the karyotype and G band patterns were analyzed after being dyed. The current results show that the number of chromo- somes in Microtus limnophilus is 2n--38. Male＇s karyotype is consist of 18 pairs of chromosomes and one pair of heterogametic sex chromosomes XY, and female＇s karyotype is consist of 18 pairs of chromosomes and one pair of homogametic sex chromosomes XX. No. 1-9 and Y chromosomes are the telocentrics chromosomes in Microtus lim- nophilus. No. 10 chromosome is subtelocentrics （st） chromosome. No. 11-18 and X chromosomes are metacentrics （m） chromosomes. The 19 pairs of chromosomes have 370 G-bands （male^365） totally. The number of the chromosomes on Microtus limnophilus and the G banding patterns are different from other breeds.