基于β-葡萄糖苷酶活力的蜂蜜真伪鉴别

Discrimination between Authentic and Fake Honey Based on β-Glucosidase Activity

提出以β-葡萄糖苷酶活力为指标的蜂蜜真伪鉴别新方法,采用分光光度法分析不同来源蜂蜜中的β-葡萄糖苷酶活力,研究不同储存条件下蜂蜜中β-葡萄糖苷酶活力的变化规律,探讨掺入不同比例大米高果糖浆的蜂蜜中以β-葡萄糖苷酶活力的变化规律。结果表明:不同来源蜂蜜中均能检测出β-葡萄糖苷酶活力,而大米高果糖浆中检测不到该酶活力;不同浓度油菜蜜分别在-18、4、25(室温)、30℃条件下储存11个月,随着储存时间的延长,蜂蜜中β-葡萄糖苷酶活力变化较小;随着油菜蜜中大米高果糖浆的掺假比例增大,β-葡萄糖苷酶活力呈线性下降。因此,β-葡萄糖苷酶活力是检测蜂蜜是否掺假的一个有效定性指标。

A new method using β -glucosidase activity was proposed to detect honey adulteration. The activities of β- glucosidase in honey from different plants, different storage conditions, and different proportions of high-fructose rice syrup were determined. The results showed that β-glucosidase activity was detectable in honey samples from different plants; however, no β-glucosidase activity was detected in honey adulterated with high-fructose rice syrup,β-glucosidase activity in rape honey showed little change during 11 months of storage at - 18 ℃, 4 ℃, room temperature or 30 ℃. With increasing high-fructose rice syrup adulteration, β-glucosidase activity revealed a linear decline. In conclusion, β-glucosidase activity can be considered as an effective indicator for the identification of honey adulteration.