反相高效液相色谱法测定巴沙鱼中的黄体素和玉米黄质

Determination of Lutein and Zeaxanthin in Pangasius bocouti by Reversed-Phase High Performance Liquid Chromatography

建立测定巴沙鱼(Pangasius bocouti)中黄体素和玉米黄质含量的反相高效液相色谱法,应用C30柱,以乙腈-甲醇-三乙胺(75:25:0.05,V/V)和甲基叔丁基醚-三乙胺(100:0.05,V/V)为流动相进行梯度洗脱,紫外检测器在450 nm处进行检测,外标法定量。结果表明:黄体素和玉米黄质线性关系良好,相关系数均为0.999 9,加标回收率分别为85.43%～96.60%和83.18%～96.18%,相对标准偏差分别为5.01%～7.15%和4.60%～8.43%,检出限分别为0.008mg/kg和0.003mg/kg。该方法灵敏度高、重现性好,适用于水产品中黄体素和玉米黄质含量的检测。

A high performance liquid chromatography method was established for the determination of lutein and zeaxanthin in Pangasius bocouti. The chromatographic separation was achieved on a C30 column using a mobile phase made up of acetonitrile, methanol and spasmolytol （75：25：0.05, V/V） by gradient elution. The detection wavelength was set at 450 nm, and an external standard method was used to quantify lutein and zeaxanthin. An excellent linear relationship of this method for lutein and zeaxanthin was observed （R2 〉 0.999）. The average spike recovery rates of lutein and zeaxanthin were 85.43% -- 96.60% and 83.18% -- 96.18% with relative standard deviations of 5.01% -- 7.15% and 4.60% -- 8.43%. The limit of detection was 0.008 mg/kg for lutein and 0.003 mg/kg for zeaxanthin. This method was sensitive, repeatable, and suitable for the determination of lutein and zeaxanthin aquatic products.