基于分段式超前校正的微流控PCR温度控制器

Temperature controller of microfluidic PCR based on sectional lead correction

根据微流控聚合酶链式反应(PCR)对温度控制的要求,设计了一种分段式超前校正温度控制器。针对被控对象的动态特性,采用了超前校正改善系统性能;根据温度误差大小调节控制参数,误差较大时快速升降温,误差较小时精确控温。实验结果表明:分段式超前校正温度控制器不仅提高了系统响应速度,还减小了稳态误差,平均升温速率为4.46℃/s,平均降温速率为4.36℃/s,稳态误差不大于0.5℃,已成功用于微流控PCR实验。

According to temperature control requirement of microfluidic PCR, a sectional lead correction temperature controller is designed. Aiming at the dynamic characteristics of controlled object, the performance of system is improved by using lead correction. The parameters of controller are adjusted according to the temperature error so as to conduct fast response when temperature error is large and precise control when the error is small. Experimental resuhs show that sectional lead correction temperature controller not only improve the response rate but also reduce the steady state error. The average heating and cooling rate of the system are 4.46 ℃/s and 4.36 ℃/s respectively, steady state error is not more than 0.5℃, and the system is successfully applied in microfluidie PCR experiment.