温度预锻炼和Ca^(2+)对温度胁迫下黄连膜脂过氧化及保护酶活性的影响

Effects of Temperature Pretreatment and Ca^(2+) on Membrane-lipid Peroxidation and Protective Enzyme in Coptis chinensis Franch. under Temperature Stress

为了明确温度预锻炼和Ca2+对温度胁迫下黄连生理指标的影响,以2年生黄连为材料,以25℃为对照,设计2组试验,探索温度胁迫下黄连膜脂过氧化及保护酶活性的变化。结果表明:在低温(-5～15℃)或高温(35℃)胁迫下,黄连丙二醛(MDA)、脯氨酸(Pro)含量增加,Pr(蛋白质)含量降低;超氧化物歧化酶(SOD)和过氧化物酶(POD)活性在低温胁迫下升高,在高温胁迫下降低,而过氧化氢酶(CAT)活性在低温和高温胁迫下都升高;进行12h温度预锻炼或CaCl2溶液浇灌,可以有效降低黄连MDA和Pro增加量,促使黄连Pr含量上升、SOD和POD活性升高。在受试的3个CaCl2浓度中,以100mmol/L CaCl2对膜系统的保护作用最佳。这表明,温度预处理和Ca2+能在一定程度上降低温度胁迫对黄连的伤害,可作为提高黄连抗逆性的一种途径。

The research aims to study the effect of pretreatment and CaCl2 solution on physiologi- cal characteristics of Coptis chinensis Franch. under temperature stress. The membrane-lipid pe- roxidation and protective enzyme of two-year-old Coptis chinensis Franch. were examined under temperature stress using two group experiments with 25℃ as a control. The results indicated that the contents of MDA and Pro raised,but the contents of proteins decreased under temperature stress. The ac- tivity of SOD,POD raised at low-temperature stress （--5℃ to 15 ℃） ,hut decreased at high-temperature stress （35℃） ;CAT activity raised at --5℃ to 15℃or 35 ℃. Pretreatment and Ca2＋ treatment reduced the contents of MDA and Pro, increased the protein contents, and improved the activity of SOD and POD, especially at 100 mmol/L of Ca2＋. From the results,pretreatment and Ca2＋ treatment are useful ways to reduce the damage of temperature stress to Coptis chinensis Franch..