铜胁迫对菊芋幼叶生理生化指标的影响

Effects of Cu Treatment on Physiological and Biochemical Characters of Erusalem Artichoke(Helianthus tuberosus) Leaves

利用不同质量浓度Cu(0、5、20、40mg/L)处理菊芋幼苗,研究Cu对菊芋幼叶中过氧化物酶(POD)同工酶、株高、叶绿素含量及细胞膜通透性的影响,以期为修复Cu污染土壤提供理论依据。结果表明:Cu处理菊芋幼苗后,叶片中共有7条POD同工酶带(P1-P7),比对照(0mg/L)新增了2条(P1、P2)酶带,且随着Cu质量浓度的增加,酶带颜色加深,酶活性增强;低质量浓度的Cu(5mg/L)对菊芋幼苗的生长具有促进作用,使菊芋幼苗的株高、叶绿素含量以及细胞膜的稳定性都有所增加,而随着Cu质量浓度的继续增加,其对菊芋幼苗的株高、叶绿素合成、细胞膜的通透性都有不同程度的抑制作用,从而对植株产生不同程度的伤害。

In order to supply a theoretical evidence for repairing the heavy metal Cu polluted soil, this paper studied the effects of Cu on the peroxidase isoenzymes, plant height, chlorophyll con- tent and membrane permeability in Helianthus tuberosus leaves. The results showed that com- pared with the control, 7 bands of POD isoenzymes （P1--PT）were observed in leaves of H. tu- berosus seedlings after Cu treatment,including two new bands（P1 and P2）. The enzyme activities increased with the intensified band color when the concentration of Cu increased. Low concentra- tion Cu（5 mg/L） stimulated the growth of H. tuberosus seedlings. It significantly increased plant height,chlorophyll content and membrane permeability. The high-level Cu treatment of H. tu- berosus inhibited plant height, chlorophyll content and membrane permeability, which caused dif- ferent levels of injury.