中药降乳散对大鼠催乳素瘤c-fos表达的抑制作用

The preventive effect of Jiangrusan on expression of c-fos in rat prolactinoma

【目的】研究中药降乳散对大鼠催乳素瘤表达癌基因c-fos的抑制作用。【方法】用皮下植入雌激素的方法制备大鼠催乳素瘤模型,将成功诱发出催乳素瘤的大鼠随机分2组,分别给予安慰剂和降乳散灌胃,用药治疗4周后处死动物,垂体称重,用放免法测定血清催乳素(prolactin,PRL)水平。用反转录-PCR方法分析原癌基因c-fos mRNA水平。【结果】降乳散组血清PRL水平为(67.9±9.6)ng/ml,垂体重量为(33.7±5.0)mg均明显低于安慰剂组[血清PRL水平为(5 667.1±860)ng/ml,垂体重量为(67.1±3.5)mg(P<0.001),降乳散组c-fos mRNA水平为(2.1±0.6)较安慰剂组(6.2±0.5)明显降低(P<0.001)。【结论】降乳散具有抗高PRL血症和抑制PRL瘤生长的作用,降低原癌基因c-fos的表达水平可能是降乳散抗PRL瘤的重要机制之一。

【Objective】 To study the preventive effect of Jiangrusan on expression of c-fos in rat prolactinoma.【Methods】 To prepare prolactinoma model in rats.The Prolactinomas were divided into 2 groups at random.Jiangrusan was orally adminstrated to rats in Jiangrusan group（n = 5）.Water were adminstrated to rats in placebo group（n = 5）.After 4 weeks of treatment,all the animals were executed.Each pituitary gland was weighted.Serum prolactin（PRL） levels were measured by RIA method.C-fos mRNA levels in pituitary tissue were measured by RT-PCR method.【Results】 The weight of pituitary gland and PRL level in Jiangrusan group were individually lower than those in placebo group（P 〈0.001 repectively）.The expression level of c-fos mRNA in Jiangrusan group was obviously lower than that in placebo group（P 〈 0.001）.【Conclusion】 Jiangrusan has potential preventive effect on estrogen-induced rat prolactinoma.The decrease of c-fos mRNA level may be involved in the mechanism of anti-prolactinoma effect of Jiangrusan.