p53和14-3-3σ在宫颈癌及宫颈上皮内瘤变中的表达和意义

Expressions and significance of p53 and 14-3-3σin human cervical cancer and cervical intraepithelial neoplasia

目的探讨p53和14-3-3σ在宫颈癌及宫颈上皮内瘤变中的表达。方法采用免疫组化SP法和RT-PCR方法检测慢性宫颈炎20例、宫颈上皮内瘤样病变(CIN)75例(其中CINⅠ、Ⅱ、Ⅲ级病变各25例)、宫颈癌患者30例组织中p53和14-3-3σ的表达。结果慢性宫颈炎组未见P53阳性表达,宫颈癌组P53阳性表达率明显高于宫颈上皮内瘤样病变组(p<0.05)。在宫颈癌组、宫颈上皮内瘤样病变组4-3-3σ阳性表达率明显低于慢性宫颈炎(P<0.05),宫颈癌组4-3-3σ阳性表达率明显低于宫颈上皮内瘤样病变组(P<0.05)。而P53和14-3-3σ在宫颈上皮内瘤样病变各分级中的阳性表达率差异无统计学意义(P>0.05)。宫颈癌p53和14-3-3σ阳性表达与宫颈癌的组织分化程度有关(P<0.05),而与临床分期及有无淋巴结转移无关(P>0.05),且二者在宫颈癌组织中的表达呈负相关(r=-0..533,P<0.05)。结论 p53和14-3-3σ的异常表达可能在子宫颈的恶性转变和细胞分化中发挥着重要的作用,提示14-3-3σ可能是宫颈癌发生和发展中的重要抑制因子。

Objective To study the expression of p53 and 14-3-3crin the chronic cervicitis,cervical intraepithelial neoplasia and cervical cancer.Methods Immunohistochemical staining SP method and RT-PCR were used to detect the expression of p53 and 14-3-3o~ in 20 cases of the chronic cervicitis,75 cases of the CIN（25 cases of the CINI,25 cases of the CINII and 25 cases of the CIN III）,and 30 cases of the cervical cancer.Results The expressions of p53 in chronic cervicitis were not detected.The expressions of p53 in cervical cancer were significant higher than that in intraepithelial neoplasia（P 0.05）.There were significant differences between the expression of p53 and 14-3-3σin cervical intraepithelial neoplasia and cervical cancer（P 0.05）.Negative correlation was also found between p53 and 14-3-3σexpression（r = -0.533,P 〈0.05）. Conclusion Abnormal expression of p53 and 14-3-3σmay contribute to the pathogenesis and development of the cervical cancer.Therefore 14-3-3cr maybe an important suppressor fat-tor in the carcinogenesis and development of cervical cancer.