氧化亚铁硫杆菌生长动力学参数

Kinetic parameters for growth of Thiobacillus ferrooxidans

基于Monod方程 ,推导并建立了氧化亚铁硫杆菌TD 10在含亚铁培养基中的生长响应模型。研究了以亚铁离子的氧化来表征氧化亚铁硫杆菌的生长过程 ;利用该模型 ,通过计算机拟合 ,确定了模型中细菌生长动力学参数 ,并用该模型预示了最大比生长速率 μm,营养物饱和常数K及细菌的初始浓度与得率系数的比值 ρ0b/Y变化时对细菌生长的影响。考察了不同二价铁离子初始浓度、温度和接种量时亚铁离子浓度的变化 ,从而确定了各实验条件下的 μm,K及ρ0b/Y值。

Based on Monod formula, a responsive mathematical model for a certain kind ofThiobacillus ferrooxidans named TD 10 growing in the substrate containing ferrous ion was built up. With computer simulating variation of ferrous ion concentration during the bacteria growth, this model can be used to determine the kinetic parameters of bacteria growth, and the influence of these parameters on bacteria growth can be predicted. Under the varied experimental conditions of initial ferrous ion concentration, temperature and inoculation amount, the ferrous ion concentration was examined, and the experimental data were simulated by computer to determine μ m, K and ρ 0 b/Y. Furthermore, the influence of experimental conditions on these parameters was discussed. [