HPLC法测定交通心肾胶囊中盐酸小檗碱含量

Determination of berberine hydrochloride in Jiaotongxinshen capsules by HPLC

目的:建立中成药交通心肾胶囊中盐酸小檗碱的含量测定方法。方法:采用ODSC18色谱柱(150mm×4.6mm,5μm),以乙腈-水(1000ml中含磷酸二氢钾3.4g和十二烷基硫酸钠1.7g)(3∶4)为流动相;流速1.0ml/min,于345nm波长处测定。结果:盐酸小檗碱在0.2～1.0μg范围内,均与相应的峰面积呈良好线性关系(r=0.9998),平均回收率为101.30%,RSD为1.44%(n=6)。结论:该方法准确可靠,可用于本制剂的质量控制。

Objective ： To establish an HPLC method for the determination of berberine hydrochloride in Jiaotongxinshen capsules. Methods ： HPLC was performed on an ODS C18 column （150 mm×4.6 mm, 5 μm） with mobile phase of acetonitrile-water （3 ： 4） at flow rate of 1.0 ml/min and the detection wavelength of 345 nm. Results ： The calibration curve for the determination of berberine hydrocbloride was linear over the range of 0.2-1.0 μg （r=0.999 8 ） and the average recovery was 101.30% （RSD=1.44%, n=6）. Conclusion ： The method is simple, rapid and accurate and can be used for quality control of Jiaotongxinshen capsules.