美味黑粉菌冬孢子萌发条件的研究

Research on Factors Influencing Teliospore Germination of Ustilago esculenta

研究了疏亲水界面、温度、pH值和不同物质对美味黑粉菌冬孢子萌发的影响,以探讨美味黑粉菌冬孢子在自然生境条件萌发的适应性。研究结果表明,疏水界面促进美味黑粉菌冬孢子萌发,在疏水的聚苯乙烯塑料培养皿和茭白茎表皮上,萌发率高;冬孢子萌发的最适温度为27～30℃,温度过高或过低均抑制萌发;酸性条件促进冬孢子萌发,最适pH值为3～4;蔗糖促进冬孢子萌发,且以1%浓度最适,但同样浓度的甘露醇、葡萄糖、海藻糖、D-果糖等抑制冬孢子萌发。

We investigated the factors influencing teliospore germination of Ustilago esculenta, such as surface characters, temperature, pH value and stimulants. The results showed that hydrophobic surfaces promoted the germination of tellospore, and the germination rates were higher on the hydrophobic polyethylene Petri dishes and surfaces of stem epidermis of Zizania latifolia. The highest germination rate occurred at 27 to 30℃, whereas it was inhibited by higher or lower temperature. The acidic condition promoted the germination of teliospore, with the optimum pH value of 3 to 4, and sucrose with the concentration of 1% was best fit for the germination of teliospore, but other carbon sources, such as mannitol, glucose, trehalose, D-fructose, inhibited teliospore germination.