抗柯萨奇B病毒性心肌炎胶囊醇提物大孔树脂纯化工艺优选

Optimization of Purification Technology for Alcohol Extract of K-CoxB-JN by Macroporous Resin

目的:优选抗柯萨奇B病毒性心肌炎胶囊醇提物大孔树脂纯化工艺。方法:以总皂苷、总黄酮和总木脂素含量为指标,考察树脂的吸附和解吸能力,筛选大孔树脂型号,采用单因素试验对最大上样量、上样流速、洗脱溶剂等进行考察。结果:非极性D101型大孔树脂效果最佳,优选的纯化工艺条件为醇提液生药质量浓度0.5 g.mL-1,药材-湿树脂量1∶4,吸附流速2 BV.h-1,3 BV水洗除杂,4 BV 70%乙醇洗脱。在此工艺条件下,总皂苷、总黄酮和总木脂素纯度分别由5.36%,0.3%,2.26%提高到53.82%,3.01%,22.87%。结论:D101型大孔树脂纯化抗柯萨奇B病毒性心肌炎胶囊醇提物,方法简便可行,纯化效果好,可推广使用。

Objective： To optimize purification technology of total saponins, flavonoids and lignins from K-CoxB-JN by macroporous resin. Method： Taking the contents of total saponins, flavonoids and lignins as indexes, adsorption and desorption properties of 10 kinds maeroporous resins were compared, based on these, maximum sample volume, sample flow rate and elution solvent were investigated by single-factor test. Result： D101 macroporous resin gave good static-dynamic and adsorption-desorption characteristics for all components, optimized purification technology was： crude drug concentration of alcohol extraction solution 0.5 g ·mL^（-1）, herbs-wet resin 1： 4, adsorption flow rate 2 BV · h^（-1）, washed with 3 BV distilled water, 4 BV of 70% ethanol as elution solvent. Under these conditions, purity of total saponins, total flavonoids and total lignins were enriched from 5.36% , 0.3% , 2.26% to 53.82% , 3.01% , 22. 87%. Conclusion： This optimum purification technology of K-CoxB-JN was simple, stable and effective with good purification effect, it could be promoted in large-scale production.