γ-巯丙基硅键合胶脱除黄连药液中重金属

Removal of Heavy Metals from Aqueous Solution of Coptidis Rhizoma by γ-Mercaptopropyl-Modified Silica Gel

目的:评价γ-巯丙基键合硅胶(MPS)脱除含生物碱类成分的中药提取液中重金属的可行性。方法:以黄连为研究对象,通过考察MPS用量、流速、径高比及温度对镉脱除效果的影响,优选脱除实验条件;以黄连药液重金属脱除前后HPLC指纹图谱相似度及含固量变化为指标,评价黄连药液重金属脱除前后化学成分的变化。结果:药材量与MPS用量比40∶1,径高比1∶3,流速10 BV.h-1,室温下,MPS对黄连药液中镉、铜的脱除率分别为81.3%,35.9%,镁、锌等金属元素的含量基本未发生变化;重金属脱除前后黄连药液HPLC指纹图谱相似度达0.99,含固量减小1.3%。结论:MPS可以有效地脱除黄连药液中重金属,且化学成分未发生明显变化。

Objective： To evaluate the feasibility of removing heavy metal ions from the extract of Chinese herbal medicines containing alkaloids by T-mercaptopropyl-modified silica gel （MPS）. Method： Some influence factors on removal of cadmium ion in the aqueous extract of Coptidis Rhizoma were studied, such as the amount of MPS, flow velocity, ratio of column diameter to height and temperature. The chemical changes were evaluated by the HPLC fingerprint profiles and dried extract content, before and after the removal of cadmium ion. Result： The optimum parameters were as follows： the ratio of medicinal herb to MPS 40： 1, 1：3 of ratio of diameter to height, 10 BV flow velocity, and the room temperature. The removing rate of cadmium ion and copper ion reached 81.3% and 35.9% , respectively. The content of magnesium and zinc ion hardly changed. Dried extract content of aqueous solution of Coptidis Rhizoma decreased by 1.3% , and the similarity of HPLC fingerprint profiles was 0.99. Conclusion： MPS can remove efficiently heavy metal ions and there was no significant change on the chemical components of aqueous solution of Coptidis Rhizoma.