响应面法优选黄芪提取工艺

Optimization of Extration Technology for Astragaloside Ⅳ from Asrtagalus membranacevs by Response Surface Methodology

目的:响应面法优选黄芪的渗漉提取工艺。方法:采用三因素三水平Box-Behnken试验设计,以乙醇体积分数、流速、乙醇用量为自变量,黄芪甲苷提取率为因变量,HPLC-ELSD测定黄芪甲苷含量,通过对自变量各水平的多元回归及二项式拟合,用响应面法优选乙醇渗漉提取工艺,并进行预测分析。结果:最佳提取工艺条件为30%乙醇,流速1.9 mL.min-1.cm-2,乙醇用量为药材量6.5倍。在此条件下,黄芪甲苷提取率理论值1.50 mg.g-1,实测值1.487 mg.g-1,说明该优选工艺模型拟合度良好。结论:Box-Behnken试验设计可用于黄芪渗漉提取工艺的优选,该优选工艺简便、稳定。

Objective： To optimize extraction technology of astragaloside 1V from Astragalus membranaceus by response surface methodology （RSM）. Method： Three-factor and three-level Box-Behnken experimental design was employed with ethanol concentration, flow rate and liquid-solid ratioas independence variables, extraction rate of astragaloside IV as dependent variable which was determined by HPLC-ELSD, experimental data obtained by multiple regression and binomial fitting of levels from independent variables, ethanol pereocation extraction technology was optimized by RSM, and to predictive analysis. Result： Optimum extraction conditions were as follows： 30% ethanol as solvent, flow rate 1.9 mL ·min^（-1）·cm^（-2）, liquid-solid ratio 6.5 mL·g^（-1） Under these conditions, theoretical value of extraction rate of astragaloside IV 1.50 mg ·g^（-1）, but measured value of 1.487 mg ·g^（-1）, it showed optimized technology model fitted well. Conclusion： Box-Behnken experimental design could be used for extraction optimization of percocation extraction process for A. membranaceus, optimized technology was simple and stable.