摘要
目的:响应面法优选黄芪的渗漉提取工艺。方法:采用三因素三水平Box-Behnken试验设计,以乙醇体积分数、流速、乙醇用量为自变量,黄芪甲苷提取率为因变量,HPLC-ELSD测定黄芪甲苷含量,通过对自变量各水平的多元回归及二项式拟合,用响应面法优选乙醇渗漉提取工艺,并进行预测分析。结果:最佳提取工艺条件为30%乙醇,流速1.9 mL.min-1.cm-2,乙醇用量为药材量6.5倍。在此条件下,黄芪甲苷提取率理论值1.50 mg.g-1,实测值1.487 mg.g-1,说明该优选工艺模型拟合度良好。结论:Box-Behnken试验设计可用于黄芪渗漉提取工艺的优选,该优选工艺简便、稳定。
Objective: To optimize extraction technology of astragaloside 1V from Astragalus membranaceus by response surface methodology (RSM). Method: Three-factor and three-level Box-Behnken experimental design was employed with ethanol concentration, flow rate and liquid-solid ratioas independence variables, extraction rate of astragaloside IV as dependent variable which was determined by HPLC-ELSD, experimental data obtained by multiple regression and binomial fitting of levels from independent variables, ethanol pereocation extraction technology was optimized by RSM, and to predictive analysis. Result: Optimum extraction conditions were as follows: 30% ethanol as solvent, flow rate 1.9 mL ·min^(-1)·cm^(-2), liquid-solid ratio 6.5 mL·g^(-1) Under these conditions, theoretical value of extraction rate of astragaloside IV 1.50 mg ·g^(-1), but measured value of 1.487 mg ·g^(-1), it showed optimized technology model fitted well. Conclusion: Box-Behnken experimental design could be used for extraction optimization of percocation extraction process for A. membranaceus, optimized technology was simple and stable.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第18期34-37,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省科技计划粤港关键领域项目(2009A030901011)