HPLC测定全蚕粉中槲皮素和山奈素的含量

Content Determination of Quercetin and Kaempferol in Silkworm Powder by RP-HPLC

目的:建立全蚕粉中槲皮素、山奈素的含量测定方法。方法:采用反相高效液相色谱法(RP-HPLC),色谱柱为KromasiL-C18柱(4.6 mm×250 mm,5μm),柱温为室温,流动相甲醇-0.4%磷酸(50∶50),流速1.0 mL.min-1,检测波长360 nm,进样量10μL。结果:槲皮素和山奈素得到良好分离,分别在0.012 5～0.400 0μg(r=0.999 8),0.014 0～0.448 0μg(r=0.999 9)呈良好的线性关系,平均回收率分别为97.53%(RSD 1.9%),100.3%(RSD 1.0%)。结论:该方法快速准确,可满足定量分析的需要。

Objective：To establish assaying methods for the determination of quercetin and kaempferol in Silkworm Powder.Method：The content of quercetin and kaempferol was determined by RP-HPLC,using a KromasiL-C18 column（4.6 mm×250 mm,5 μm）.The column temperature was fixed on room temperature.Methanol-0.4% phosphoric acid（50∶50） was used as mobile phase,flow-rate was 1.0 mL · min-1.The detecting wavelength was set at 360 nm.Result：The linear range were 0.012 5-0.4 μg（r=0.999 8） and 0.014-0.448 μg（r=0.999 9）.The average recoveries were 97.53% and 100.3%,with the RSD of 1.9% and 1.0%.Conclusion：The method established is rapid and accurate,which could be used for quality control of Silkworm Powder.