麻杏石甘汤对LPS诱导的大鼠发热模型的影响

Antifebrile Effect of Maxing Shigan Tang on Fever Induced by LPS in Rats

目的:建立静脉注射内毒素的大鼠发热模型,观察麻杏石甘汤的解热作用。方法:大鼠尾静脉注射多个浓度(5,10,20,40,60,80μg.kg-1)的内毒素,观察各组大鼠的体温变化,建立适宜的尾静脉注射脂多糖(LPS)发热模型;将大鼠随机分为空白组,模型组,给药组(低、中、高剂量分别为1.33,4.5,15.2 g.kg-1),实验当天灌胃给药后立即造模,观察麻杏石甘汤对该发热模型动物体温变化的影响。结果:静脉注射内毒素可引起SD大鼠显著升温(P<0.01),结果表明静脉注射内毒素致SD大鼠发热模型最佳造模剂量为20μg.kg-1。灌服低、中、高剂量麻杏石甘汤的大鼠的体温均较模型组明显降低(P<0.05)。比较各组的体温反应指数TRI值,发现各组的解热效应有明显的剂量效应关系。结论:麻杏石甘汤对静脉注射LPS诱导的大鼠发热模型具有较好的解热作用,且呈剂量依赖性。

Objective：Using rat intravenous endotoxin model of fever to observe the antifebrile effect of Maxing Shigan Tang.Method： the effects of Maxing Shigan Tang on body temperature was observed on the fever induced by intravenous injection of lipopolysaccharide（LPS） at the dose 5,10,20,40,60,80 μg · kg-1,respectively.The rats were randomly divided into blank group,model group,treatment group（low,medium and high dose respectively 1.33,4.5,15.2 g · kg-1）,the model was induced immediately after administration.Result： Intravenous injection of endotoxin could increase body temperature significantly（P〈0.01）.The results showed that the dose of intravenous injection of endotoxin induced SD rat fever model was 20 μg · kg-1.The body temperature was significantly lower compared with model group after fed with low,medium and high dose of Maxing Shigan Tang（P〈0.05）.TRI showed a clear dose-response relationship.Conclusion： Maxing Shigan Tang has a better antifebrile effects on the fever induced by intravenous injection of LPS in rats,and show a dose-dependent manner.