蛇黄乳膏止痒功效相关机制

Antipruritic Mechanism of Shehuang Cream in Animal

目的:探讨蛇黄乳膏防治湿疹止痒作用的相关机制。方法:取小鼠随机分组,即对照组、模型组、艾洛松组、蛇黄乳膏各浓度组,采用2,4-二硝基氟苯(DNFB)致小鼠迟发性变态反应;用二甲苯致小鼠耳廓肿胀计算出肿胀抑制率;用角叉菜胶致小鼠足肿胀观察足肿胀改善情况;复制冰醋酸致小鼠毛细血管通透性增加模型,以上实验均于皮损约4cm2处外用给药1 mg.g-1。取SD大鼠60只,随机分为6组,对照组、模型组、氯雷他定组、蛇黄乳膏各浓度组,给药5 d,用0.1%磷酸组胺复制大鼠皮肤通透性增高模型;取豚鼠65只,分5组,外用皮损3 cm2给药5 d,用磷酸组胺致豚鼠瘙痒统计致痒浓度值。通过以上实验来探讨蛇黄乳膏止痒作用的相关机制。结果:蛇黄乳膏可显著抑制DNFB所诱导迟发性超敏反应,但对胸腺指数以及脾脏指数影响不明显;蛇黄乳膏可直接对抗组织胺所诱导的瘙痒反应(P<0.01),并可降低磷酸组胺所致皮肤通透性增加(P<0.01);对二甲苯直接刺激血管扩张所致的炎症、冰醋酸通过刺激炎症介质生成等所致的炎症反应有明显抑制作用(P<0.01),但对有多种致炎因子参与的角叉菜胶所致的炎症反应作用微弱。结论:蛇黄乳膏的止痒作用与调节机体免疫功能、直接对抗组织胺、减少炎症反应等作用有关。

Objective：To study the mechanism of Shehuang cream on prevention and curing of eczema.Method：Seventy male mice were randomized to control group,model group,Asmanex group,different concentration groups of Shehuang cream,mice delayed hypersensitivity reaction was induced by dinitrofluobenzene;mice pinna and paw swelling were induced by carrageenan to investigate inhibition rate on tumefaction,and the glacial acetic acid induced capillary permeability model was used to evaluate the effect of Shehuang cream and explore it’s mechanism.Sixty SD rats were randomized to control group,model group,Ioratadine group,different concentration groups of Shehuang cream,0.1% phosphoric acid histamine was used to detect the degree of clarification.Sixty-five guinea-pigs were divided into differents groups,guinea pig pruritus were induced by histamine phosphate.Result：Shehuang cream could significantly inhibit the delayed hypersensitivity induced by 2,4-dinitrofluorobanzene（DNFB）,but has no effect on the indexes of thymus and spleen in mice.Shehuang cream could relieve the itch responses in guinea pig and skin permeability in rat induced by histamine（P〈0.01）;it also inhibited the auricula inflammation induced by of xylene and enterocoelia capillary permeability by glacial acetic acid in mice（P〈0.01）,but had a little action on the paw swelling induced by carrageenan in mice.Conclusion：The results indicte that Shehuang cream’s antipruritic effect may be related to regulation of immune function,antagonistic action on histamine and reducing the inflammatory reaction.