纤体降脂Ⅰ号预防大鼠去卵巢后肥胖

Prevention of Xianti JiangzhiⅠPrescription on Obeseing Induced by Ovariectory in Rats

目的:研究纤体降脂Ⅰ号对大鼠去卵巢后肥胖的预防作用,探讨其预防肥胖的作用机制。方法:270 g左右的SD雌性大鼠70只,按体重随机选出假手术组10只,其余采用双侧去卵巢法,建立去卵巢大鼠模型。造模成功后,将大鼠随机分为模型对照组、纤体降脂Ⅰ号高剂量组(13.2 g.kg-1.d-1)、中剂量组(6.6 g.kg-1.d-1)、低剂量组(3.3 g.kg-1.d-1)、曲美组(8.9×10-4g.kg-1.d-1)、己烯雌酚组(2.2×10-5g.kg-1.d-1),开始灌胃给药干预,连续3周,实验结束后,从大鼠体重、腹脂、Lee’s指数、血脂等方面评价其药效;从血清雌二醇和瘦素含量以及下丘脑瘦素的表达,探讨其作用机制。结果:纤体降脂Ⅰ号能明显抑制模型鼠体重和食量的增长(P<0.05或P<0.01),降低腹脂含量和Lee’s指数(P<0.05或P<0.01),有效预防大鼠去卵巢后肥胖;显著降低大鼠血清甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)水平(P<0.05或P<0.01),有升高高密度脂蛋白胆固醇(HDL-C)的趋势;能明显提高大鼠血清雌二醇水平(P<0.05或P<0.01)。结论:纤体降脂Ⅰ号能够有效控制大鼠体重和食欲,预防大鼠去卵巢后肥胖的发生,降低血脂;提高血清雌激素含量,这可能是其预防大鼠去卵巢后肥胖的机制之一。

Objective：In order to investigate the effects of Xianti JiangzhiⅠPrescription in proventing obeseing induced by ovariectory in ratsand explore the possible mechanism.Method：Seventy female SD rats,weighting about 270 g were randomly divided into 7 groups：sham-operated group（sham）,simple ovariectomized group and three treated groups with low（3.3 g · kg-1 · d-1）,middle（6.6 g · kg-1 · d-1） and high（13.2 g · kg-1 · d-1） dosage of Xianti JiangzhiⅠprescription,diethyllstillbestrol group（2.2×10-5g · kg-1 · d-1）,trimetazidine group（8.9×10-4g · kg-1 · d-1）,ten each group.The administration started 10 days after operation and continued for 3 weeks.In the end,the blood samples were collected to measure following indexes：serum estradiol（E2）,blood lipids[total cholesterol（TC）,trigly cerides（TG）,high density lipoprotein cholesterol（HDL-C）,low-density lipoprotein cholesterol（LDL-C）],leptin,wet weight of fat,et al.Result：Xianti JiangzhiⅠprescription can significantly inhibit the increase of weight and food intake in the model rats（P〈0.05 or P〈0.01）,decreased the weight of wet abdominal fat,reduce the lees indexes（P〈0.05 or P〈0.01）,significantly prevented obesity induced by ovariectory,significantly decreased the TG,TC,LDL-C level（P〈0.05 or P〈0.01）and increased the level of blood estrogen（P〈0.05 or P〈0.01）.Conclusion：Xianti JiangzhiⅠprescription can significantly prevent obesity induced by ovariectory by;decreasing the blood lipids and increasing the level of blood Estrogen,this may be one of its mechanisms.