桃叶鸦葱鞣质的提取及体外抗氧化作用

Extraction and Antioxidative Activity of Tannins from Scorzonera Sinensis

目的:测定桃叶鸦葱根及地上部分中鞣质的含量,考察鞣质抗氧化活性。方法:采用正交设计优化鞣质提取工艺,采用络合滴定法测定鞣质含量。采用碘量法测定0.01～0.05 g.mL-1鞣质对过氧化氢(H2O2)的作用,采用分光光度法测定其对羟自由基(.OH)的作用。结果:桃叶鸦葱根及地上部分中鞣质含量分别为8.18%和9.27%。桃叶鸦葱鞣质的体外抗氧化具一定的剂量依赖性,质量浓度为0.05 g.mL-1时对H2O2及.OH的平均清除率分别达到8.89%,88.24%。结论:桃叶鸦葱鞣质有一定抗氧化作用。

Objective：To determine the content of tannins in above and under ground parts of scorzonera sinensis.To observe antioxidative activity of tannin from scorzonera sinensis.Method：The complexometric titration was used to determine the content of tannins.Iodometric and spectrophotometry were used to determine the effects of tannins on H2O2 and · OH.Result：The tannins in above and under ground parts of scorzonera sinensis was 8.18% and 9.27% accordingly.With the Concentration of 0.05 g · mL-1,the clearance rates on H2O2 and · OH were 8.89% and 88.24%.Conclusion：Tannins of scorzonera sinensis have a strong ability to resistance oxidation