丁基苯酞对慢性脑缺血大鼠认知和脑组织形态的改善

Effects of butylphthalide on the cognition and brain tissue morphous of chronic global brain ischemic rats

目的观察丁基苯酞对慢性脑缺血大鼠认知和脑组织神经元形态异常的改善作用。方法持久性结扎大鼠两侧颈总动脉3个月,制备慢性脑缺血模型。实验分4组,每组8只:假手术组、模型组、丁基苯酞30和120 mg/kg组。造模后第46天开始给药,每天1次,连续45 d后,用水迷宫定位航行和空间探索实验检测大鼠的空间认知能力。同时,取皮层和海马,用HE染色法观察神经元形态。结果水迷宫定位航行实验中,随着训练天数的增加,模型组逃避潜伏期逐渐长于假手术组;与模型组比较,丁基苯酞两个组的潜伏期逐渐缩短。空间探索实验显示,模型组大鼠的目标象限活动时间明显少于假手术组(P<0.01),而与模型组比较,丁基苯酞2个剂量明显延长大鼠的目标象限活动时间(P<0.05)。同时,HE染色显示模型组大鼠脑组织神经元形态明显异常,而该药对神经元形态异常有明显的改善。结论丁基苯酞能够改善慢性脑缺血大鼠的认知缺陷,并改善脑组织神经元的形态异常。

Objective To investigate the effects of butylphthalide on the cognition and the morphology of neuron in brain tissues of chronic cerebral isehemic rats. Methods The rats were divided into four groups （n = 8 in each group） ：sham group, model group and butylphthalide 30 and 120 mg/kg groups. Chronic cerebral isehemie rat models were induced by ligating the bilateral common carotid arteries for three months. At day 46 after operation, rats were administered with vehicle or butylphthalide once a day for 45 d consecu- tively. And then, the spatial cognition was determined with place navigation test and spatial probe test in the water maze. The morphology of neurons in brain tissues was observed with HE staining. Results With the increase of training days, the escape latency in model group was gradually longer than that in sham group, but the escape lateneies in butylphthalide groups were shorter significantly than in model group. The time spent in the platform-quadrant in model group was less than that in sham group（ P 〈 0. 01 ） ,but it was longer in butylphthalide groups than in model group （ P 〈 0. 05 ）. Meanwhile, HE staining showed that the brain neuron was abnormal in model rats and was improved by butylphthalide. Conclusion Butylphthalide could improve the damage of cognition and alleviate their mor- phological abnormalities of brain tissues in cerebral ischemic rats.