多重周期饥饿对奥尼罗非鱼体内蛋白酶活力影响

Effects of Multiple Cycle Starvation and Re-feeding on Protease Activity of Tilapia(Oreochromis niloticus×O.Areus)

研究了多重周期饥饿对奥尼罗非鱼(Oreochromis niloticus×O.Areus.)肝胰脏及胃肠内蛋白酶活性的影响.分成4组:对照组、饥饿1 d投喂6 d(实验1组)、饥饿2 d投喂5 d(实验2组)和饥饿3 d投喂4 d(实验3组),实验持续56d.结果显示:循环饥饿处理和恢复投喂后,肝胰脏内胰蛋白酶的活力表现高度一致,实验1组均显著高于对照组和实验3组(P<0.05);胃肠内胰蛋白酶活力随循环饥饿时间的延长逐渐下降,恢复投喂后实验1组胃肠内胰蛋白酶活力显著高于其它三组(P<0.05);肝胰脏和胃肠内胃蛋白酶活力随循环饥饿时间延长均逐步下降,恢复投喂后肝胰脏及胃肠内胃蛋白酶活力从高至低依次为:实验1组、实验2组、实验3组和对照组.

A 8 weeks study was conducted to determine the effect of multiple cycle starvation and re-feeding on protease activity in hepatopancreas,stomach and intestine of talipia（Oreochromis niloticus×O.Areus）.After two weeks acclimation,180 juveniles were divided into four groups（control,1,2 and 3 respectively,three replicates,each 15 individuals）,which were cycle starved for 0 d（control）,1 d,2 d and 3 d,then cycle re-fed 7 d,6 d,5d and 4 d respectively,The results showed that the change of trypsin activity in hepatopancreas was consistency between starvation and re-feeding period,it was significantly higher in 1 group than control and 3 group（P〈0.05）.Trypin activity in stomach and intestine was decreased gradually with cycle starvation prolonged,after re-feeding trypsin activity in 1 group was significantly higher than the other three groups（P〈0.05）.Pepsin activity in hepatopancreas was gradually decreased with starvation prolonged,after re-feeding pepsin activity in hepatopancreas,stomach and intestine from high to low is as follows： group 1,group 2,group 3 and control group.