男性不育患者精液活性氧和精浆丙二醛测定临床价值研究

The clinical value of determination of reactive oxygen species and seminal plasma malondialdehyde in male sterility patients semen

目的探讨男性不育患者精液活性氧(ROS)和精浆丙二醛(MAD)测定的临床价值。方法选择有生育力的男性健康对照组50例,男性不育症组270例,化学发光法测定精液ROS,生化比色法测定精浆中的MAD。结果参数正常男性不育组和参数异常男性不育组精液ROS分别为(39.28±3.57)RLU/S和(79.77±4.32)RLU/S,均明显高于健康对照组精液ROS(17.16±2.84)RLU/S,参数异常男性不育组精浆MAD为(27.81±3.93)nmol/mL,明显高于健康对照组精浆MAD(5.40±1.12)nmol/mL。白细胞精子症组、畸形精子症组及轻度、中度、重度弱精子症组精液ROS和精浆MAD的水平明显高于健康对照组,随着精子活动力下降,精液中ROS和精浆中的MAD的水平依次升高,以上结果差异均有统计学意义(P<0.01)。结论 ROS是影响男性不育的一个重要病因和病理因素,对男性不育患者精液ROS和精浆MAD检测具有重要的临床意义。

Objective To explore the clinical value of semen ROS and seminal plasma MAD determination. Methods Fertility of male healthy control group of 50 cases,270 cases of male infertility group were selected,semen ROS was detected by chemiluminescence method, MAD in the seminal plasma was detected by biochemical colorimet- ric. Results Semen ROS of normal parameters group and abnormal parameters group were （39. 28±3. 57） and （79.77±4.32） RLU/S, respectively, these were significantly higher than that in healthy control group （17. 16±2.84）RLU/s. Abnormal parameters group seminal plasma MAD was （27.81±3.93）mmol/mL, it was significantly higher than healthy control group （5.40±1. 12）mmol/L. Semen ROS and seminal plasma MAD of Azoospermia group of white blood cells,abnormal sperm group and mild, moderate or severe asthenozoospermia groups were sig- nificantly higher than healthy controls. With the sperm motility declining, semen ROS and seminal plasma in the MAD level increased successively,the differences were statistically significant（P〈0.01）. Conclusion ROS is an im portant cause and pathological factor for affecting male infertility, semen ROS and seminal plasma MAD detection have important clinical significance in male infertility.