脑红蛋白在感染性脑水肿中的表达变化

The research of NGB expression change in infective brain edema induced by lipid polysaccharide

目的探讨脑红蛋白(NGB)在脂多糖致感染性脑水肿中的表达。方法 60只大鼠随机分为3组:脂多糖(LPS)组、生理盐水组、假手术组。分别向脂多糖组、生理盐水组大鼠颈内动脉注射LPS 150μg(0.15mL)、生理盐水0.15mL建立模型。建模后12h取大鼠脑组织采用干湿重法检测脑组织含水量,甲酰胺法检测血脑屏障通透性,反转录聚合酶链反应检测NGB mRNA含量,以及免疫组化法检测NGB蛋白表达。结果 12h后LPS组脑组织含水量82.14%±0.43%,伊文思蓝含量(10.70±0.36)μg,NGB mRNA及蛋白表达量(1.31±0.08)明显高于生理盐水组以及假手术组(P<0.05),假手术组和生理盐水组脑组织含水量、伊文思蓝含量、NGB mRNA以及蛋白表达差异无统计学意义。结论脑红蛋白在LPS致感染性脑水肿模型中明显升高,其可能为机体对感染性脑水肿的一种保护机制。

Objective To explore the expression of neuroglobin（NGB） in rats brain edema model induced by lipid polysaccharide（LPS）.Methods 60 female rats were randomly divided into 3 groups：lipid polysaccharide group（L）,saline group（S group）,Sham group（C group）.Three groups were carrotid injected with LPS 150 μg（0.15 mL）,saline 0.15 mL,nothing respectively.After 12 hours,all rats were sarcrificed,and the brain tissues were harvested.Dry-wet weight,evan blue,RT-PCR,and immunohistochemistry were used to measure the brain tissue water content,the integrity of blood-brain barrier,the gene and protein expression of NGB.Results 12 hours after the operation,the brain tissue water contents,EB value,NGB m RNA and protein expression were significantly high in LPS group,and the the brain tissue water contents,EB value,NGB m RNA and protein expression were not statistically significant between C and S group.Conclusion The expression of NGB in rats brain edema model induced by lipid polysaccharide（LPS） is obviously high,it maybe the protection mechanism of NGB in rat brain edema. 更多