XE-5000血液分析仪检测有核红细胞的评价

Evaluation on nucleated red blood cell detection by XE-5000 automated hematology analyzer

目的探讨Sysmex XE-5000血液分析仪(简称XE-5000)自动计数外周血有核红细胞(NRBC)的方法学特点,评价其临床应用价值。方法评析XE-5000重复性、线性范围、携带污染率,并对391份静脉血标本做常规测定,评价其对NRBC的报警、定量测定,并与手工涂片法进行对照。结果 XE-5000测定NRBC的重复性较好,线性范围较广,携带污染率较小。当NRBC比例>5.0%时,则引起白细胞计数和淋巴百分比计数假性增多。以显微镜复检为金标准,XE-5000与显微镜计数法计数外周血中NRBC数量有极好的相关性(r=0.997),其NRBC报警的敏感性为100.0%,特异性为85.4%。结论 XE-5000可灵敏、准确、精密地自动计数外周血中NRBC,适合临床检测NRBC。

Objective To evaluate the clinical application significance and methodology characteristic of Sysmex XE-5000 automated hematology analyzer（XE-5000） to count nucleated red blood cell（NRBC）.Methods The repeatability,linear range and carryover rate of XE-5000 were evaluated.A total of 391 blood samples were measured,and the alarm and quantitative determination of XE-5000 for NRBC were evaluated.The results were compared with manual microscopy.Results The XE-5000 results showed good repeatability,linear range and low carryover rate.When NRBC% 〉5.0%,it caused false increasing of leucocyte and lymphocyte.The results of manual microscopy were as a gold standard.The correlation was good between the XE-5000 and manual microscopy（r=0.997）.The sensitivity for NRBC was 100.0%,and the specificity was 85.4%.Conclusions NRBC counting by XE-5000 is sensitive,accurate and reliable,which is suitable for the clinical detection of NRBC in peripheral blood.