金针菇菌株单核原生质体交配型与菌丝生长分析

Analysis of Mononuclear Protoplast Mating Type and Mycelial Growth Rate of Flammulina velutipes Strains

对3个金针菇菌株进行了原生质体分离与交配型分析,结果表明,3个金针菇菌株均出现2种交配型,2种交配型比例不一致,其中有2个菌株的两种单核体的分离比例均符合1∶1,1个菌株的两种单核体的分离比例不符合1∶1,并且在交配型比例上出现偏差现象。对3个金针菇菌株的单核原生质体菌株生长速度试验表明,单核原生质体菌株在菌丝生长速度上其出发菌株比较,表现出生长速度慢的现象,同一菌株具有2种不同交配型因子的原生质体单核菌株两两配对杂交菌株,其生长速度均快于其出发菌株和原生质体单核菌株,且同一菌株具有2种不同交配型因子的原生质体单核菌株生长速度存在不同程度的显著性差异;菌落形态和菌丝生长速度与交配型因子有关。

3 Flammulina velutipes strains were studied by protoplast isolation and mating-type analysis.Results showed that each of the 3 Flammulina velutipes strains had 2 mating types.Among them,the ratio of two mating-type monokaryons of 2 Flammulina velutipes strains was 1∶ 1,while another strain was not,and the proportion of mating types had different phenomenon.The mycelial growth tests of 3 Flammulina velutipes strains indicated that in 3 Flammulina velutipes strains,the mycelial growth rate of monokaryons was slower than that of the starting strains.Moreover,the mycelial growth rate of hybrids between two monokaryons of one strain was faster than that of theirs starting strains and monokaryons.In addition,there were significant differences in the mycelial growth rate of monokaryons.The results also indicated that the colonial morphology and mycelial growth rate had relations with mating factors.