电针联合丰富康复训练对脑损伤仔鼠脑皮质脑源性神经营养因子表达的影响

Effects of Electroacupuncture Combined with Enriched Rehabilitation Training on Expression of Brain-derived Neurotrophic Factor in Cerebral Cortex of Neonatal Rats with Brain Injury

目的探讨电针联合丰富康复训练对脑损伤仔鼠脑皮质脑源性神经营养因子(BDNF)表达的影响。方法脂多糖组孕17 d孕鼠腹腔注射脂多糖450μg/kg d,连续2 d,制备脑损伤模型;对照组孕鼠腹腔注射等量生理盐水。脂多糖组仔鼠随机分为非干预组和干预组,干预组予丰富康复训练、电针干预,每天1次,至第28天。对照组、非干预组仔鼠均常规饲养。采用SABC免疫组织化学方法检测第1、14、21、28天脑皮质BDNF阳性胞表达情况。结果第1天脂多糖非干预组仔鼠脑皮质BDNF表达高于对照组(P<0.01),此后两组间比较无显著差异(P>0.05);干预组仔鼠各时相点脑皮质BDNF表达均高于非干预组和对照组(P<0.01)。结论电针联合丰富康复训练能显著增强脑损伤仔鼠脑皮质BDNF阳性表达。

Objective To explore the effects of electroacupuncture combined with enriched rehabilitation training on the expression of brain-derived neurotrophic factor （BDNF） in the cerebral cortex of neonatal rats with brain injury. Methods Lipopolysaccharide （LPS, 450 μg/kg.d） was injected to pregnant rats on the 17th and 18th days intraperitoneally. The control group was injected with equal sterile normal saline. The neonatal rats injected with LPS were randomly divided into non-intervention group and intervention group. The intervention group received intervention once a day until the 28th day. The control group and the non-intervention groups were fed regularly, lmmunohis- tochemistry （SABC） was used to detect the expression of BDNF on the 1st, 14th, 21st and 28th days. Results The expression of BDNF was higher in the non-intervention group than in the control group （P〈0.01） on the 1st day, but there was no significant difference on the 14th, 21st and 28th days. And it was higher in the intervention group than in the other 2 groups （P〈0.01）. Conclusion Electroacupuncture combined with enriched rehabilitation training can significantly improve the expression of BDNF in cerebral cortex of neonatal rats with brain injury.