HPLC法测定人血浆中奥沙普秦浓度

HPLC with solid phase extraction assay for determ ination of oxaprozin in human plasma

目的:建立固相萃取高效液相色法测定人血浆中奥沙普秦浓度的方法,并应用于人体血浆药物浓度测定。方法:固相萃取净化和富集血浆样品。色谱柱为Kromasil C18柱,流动相为0.02 mol/L磷酸二氢钾缓冲液(pH 5.0)-甲醇(85∶15),流速为1.0 ml/min,紫外检测波长285 nm。血浆中内源性物质对样品测定无干扰。结果:本方法奥沙普秦线性范围为0.1～100μg/ml(r=0.999 2),最低定量浓度为0.1μg/ml,回收率为99.39%,日内、日间RSD均小于2%。结论:本法简便、准确,适用于奥沙普秦临床药物浓度测定。

Objective ： To establish an HPLC me thod by solid - phase extraction in order to determine of Oxaprozin in human plasma and for human drug plasma concentration determine. Methods： Proteins in plasma samples were precipitated by solid - phase extraction. Kromasil Cls was used with the mobile phase of 0.02 mol/L Nail2 P04 solution （pH 5.0） -methanol（ 85： 15 ） at a flow rate of 1.0 ml/min. The detection wavelength was at 285nm. The endogenous substance in plasma did not interfere with the determination of the samples. Results ： The linear range was 0.1 - 100 i.~g/ml （ r = 0.999 2）, and the limit of quantitation was 0.1 ixg/ml. The method recovery was 99.39%. Both within - day RSD and between - day RSD were less than 2%. Conclusion： The method is simple and accurate. It is suitable for the drug plasma concentration of oxaprozin.