蒽酮-硫酸法测定复方白及乳膏中白及多糖的含量

Content Determination of Bletilla Striata Polysaccharide in Compound Bletilla Striata Cream by Anthrone-Sulfuric Acid Method

目的:建立复方白及乳膏中白及多糖的含量测定方法。方法:以无水葡萄糖为对照品,氯仿-乙醇混合溶液为溶解介质,蒽酮-硫酸法测定,测定波长620 nm。结果:白及多糖在1.9～34.2μg/mL范围内线性关系良好,A=0.033C-0.0067(r=0.999 7),回收率为99.55%,RSD=1.12%(n=6)。结论:该方法便捷、重现性好,适用于复方白及乳膏中白及多糖的含量测定。

To establish the quantitative determination method of the bletilla striata polysaccharide in Compound Bletilla Striata Cream.Methods： Using the anhydrous dextrose as the control,the bletilla striata polysaccharide was dissolved in the mixture of chloroform and alcohol.Anthrone-Sulfuric acid method was used and the wavelength was 620 nm.Results： The bletilla striata polysaccharide curve was linear within a range of 1.9~34.2 μg/mL.Linear equation was A=0.033C-0.0067（r=0.999 7）.The average recovery was 99.55% with RSD of 1.12%（n=6）.Conclusions： This method is convenient,stable,and can be used to assay content of the bletilla striata polysaccharide in Compound Bletilla Striata Cream.