摘要
目的:建立复方白及乳膏中白及多糖的含量测定方法。方法:以无水葡萄糖为对照品,氯仿-乙醇混合溶液为溶解介质,蒽酮-硫酸法测定,测定波长620 nm。结果:白及多糖在1.9~34.2μg/mL范围内线性关系良好,A=0.033C-0.0067(r=0.999 7),回收率为99.55%,RSD=1.12%(n=6)。结论:该方法便捷、重现性好,适用于复方白及乳膏中白及多糖的含量测定。
To establish the quantitative determination method of the bletilla striata polysaccharide in Compound Bletilla Striata Cream.Methods: Using the anhydrous dextrose as the control,the bletilla striata polysaccharide was dissolved in the mixture of chloroform and alcohol.Anthrone-Sulfuric acid method was used and the wavelength was 620 nm.Results: The bletilla striata polysaccharide curve was linear within a range of 1.9~34.2 μg/mL.Linear equation was A=0.033C-0.0067(r=0.999 7).The average recovery was 99.55% with RSD of 1.12%(n=6).Conclusions: This method is convenient,stable,and can be used to assay content of the bletilla striata polysaccharide in Compound Bletilla Striata Cream.
出处
《儿科药学杂志》
CAS
2012年第9期40-43,共4页
Journal of Pediatric Pharmacy
基金
太和科研基金资助项目(2009D34)
湖北省教育厅优秀中青年基金资助项目(基金编号:Q201002110)
关键词
复方白及乳膏
白及多糖
蒽酮-硫酸法
含量测定
Compond bletilla striata cream
Bletilla striata polysaccharide
Anthrone-sulfuric acid method
Determination