NaCl胁迫对思茅松幼苗生长的影响

NaCl Stress on Seedlings Growth of Pinus kesiya var. langbianensis

[目的]以思茅松幼苗为研究对象,研究NaCl胁迫对其种子发芽和幼苗生长的影响。[方法]分别用25、50、100、200、300 mmol/L的NaCl处理种子,分析各处理下思茅松种子发芽情况,幼苗生长情况以及对4种生理生化指标的影响。[结果]在100 mmol/L盐分范围内,幼苗能正常生长,发芽率与发芽势与对照无显著差异。在100 mmol浓度下,幼苗的SOD酶含量最大。200 mmol/L浓度时,思茅松幼苗的发芽率、发芽势降低,植物的可溶性糖、叶绿素总量最大。300 mmol/L,幼苗不能正常生长,出苗死亡率高,畸形苗多。幼苗体内的MDA含量随着盐胁迫浓度的增加而增加。[结论]思茅松幼苗在100 mmol/L以内的盐分范围内能正常生长。

[ Objective ] Taking Pinus kesiya var. langbianensis as the research object, NaC1 stress on seedlings growth of Pinus kesiya var. langbianensis was studied. [ Method ] Seed germination, seedling growth and four kinds of physiological and biochemieal indexes at different concentrations such as 25, 50,100,200,300 mmol/L were studied. On 100 retool/L, the SOD in the seedlings got the max. On and below 100 mmol/L, the seedlings eould grow normally with germination rante and germination energy decreased no significant difference eompared with control. On 200 mmol/L , soluble sugar,total chlorophyl in the seedlings were the highest. On 300 mmol/L,the seedlings grew abnormally, with high malformation rate and mortality rate. The content of MDA increased with increasing of NaC1 concentration. [ Conclusion] Pinus kesiya vat. langbianensis seedling could grow normally at salt concentration less than 100 mmol/L.