摘要
从百合基因克隆(包括减数分裂基因、花发育基因、花色基因、花粉发育基因以及抗性相关基因),再生体系建立(包括不定芽再生系统、原生质体再生系统、体细胞胚再生系统、愈伤组织再生系统),外源基因转化方法(包括农杆菌介导的遗传转化、DNA直接导入系统的基因转化、花粉管通道法)及转基因应用等方面,介绍近年来国内外百合分子育种的研究进展,并讨论了百合分子育种研究中存在的问题和应用前景。
New advances in molecular breeding of lily were reviewed in this paper. The first part is the gene cloning including meiosis genes, flower development genes, floral color genes, pollen development genes, and genes related to resistance. The second part is the establishment of regeneration system including adventitious shoot regeneration system, protoplast regeneration system, somatic embryo regeneration system, and callus regeneration system. The third part is gene transformation methods including Agrobacterium mediated genetic transformation, DNA directly into the system, gene transformation via pollen-tube pathway. The fourth part is the trangene application in lily. Meanwhile, the existing problems and prospects of the application of molecular breeding in lily were discussed.
出处
《园艺学报》
CAS
CSCD
北大核心
2012年第9期1793-1808,共16页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31071815)
教育部博士点基金项目(20110014110006)
国家‘十二五’‘863’计划项目(2011AA100208)
关键词
百合
分子育种
花发育
基因克隆
lily
molecular breeding
floral developmentgene
genecloning
